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Induction charging and electrostatic classification of micrometer-size particles for investigating the electrobiological properties of airborne microorganisms.

Authors
Mainelis-G; Willeke-K; Baron-P; Grinshpun-SA; Reponen-T
Source
Aerosol Sci Tech 2002 Apr; 36(4):479-491
NIOSHTIC No.
20022255
Abstract
Our earlier studies have shown that the electrostatic collection technique, a potentially "gentle" bioaerosol collection method, allows for efficient collection of airborne bacteria, but sensitive bacteria such as Pseudomonasfluorescens (P.fluorescens) lose their culturability during collection. We hypothesized that excessive stress was imposed on the sensitive bacteria by the sampler's conventional corona charging mechanism. In this research, we developed and built an experimental setup that allows us to analyze electro-biological properties of airborne microorganisms. In this experimental system, we imparted electric charges on airborne biological and nonbiological particles by aerosolizing them in the presence of an electric field. The charged P. fluorescens test bacteria and NaCI test particles were then channeled into a parallel plate mobility analyzer, which we have designed so that bacteria and inert particles carrying specific charge ranges can be extracted and made available for further analysis. When testing the experimental system, we related the extracted particle concentrations to the total particle concentration and obtained the charge distributions of these particles at different charging conditions. Our results have ( shown that even without charging, aerosolized P. fluorescens bacteria have a net negative charge and can carry up to 13,000 elementary charges per bacterium. In contrast, the NaCI particles were found to carry very few electric charges. We concluded that the electric charge carried by a bacterium consists of 2 components: its own natural charge, which can be high, and the charge imposed on it by the dispersion process. Our experiments have shown that the charge distributions on biological and non biological particles can be effectively manipulated by varying the external electric field during their aerosolization. Since airborne microorganisms may carry high internal electric charges, their collection by electrical field forces may be possible without first electrically charging them.
Keywords
Air-sampling; Air-sampling-techniques; Electrical-fields; Aerosol-generators; Kinetics; Air-sampling-equipment; Microorganisms; Airborne-particles; Aerosols; Electrical-charge
Contact
Department of Environmental Sciences, Rutgers, The State University of New Jersey, 14 College Farm Road, New Brunswick, NJ 08902
CODEN
ASTYDQ
Publication Date
20020501
Document Type
Journal Article
Email Address
mainelis@envsci.rutgers.edu
Funding Amount
933298
Funding Type
Grant
Fiscal Year
2002
NTIS Accession No.
NTIS Price
Identifying No.
Grant-Number-R01-OH-003463
Issue of Publication
4
ISSN
0278-6826
NIOSH Division
DART
Priority Area
Research Tools and Approaches: Exposure Assessment Methods
Source Name
Aerosol Science and Technology
State
NJ; OH
Performing Organization
University of Cincinnati
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