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The effect of diesel exhaust particles (DEP) and carbon black (CB) on thiol changes in pulmonary ovalbumin allergic sensitized brown Norway rats.

Authors
Al-Humadi-NH; Siegel-PD; Lewis-DM; Barger-MW; Ma-JYC; Weissman-DN; Ma-JKH
Source
Exp Lung Res 2002 Jul-Aug; 28(5):333-349
NIOSHTIC No.
20022189
Abstract
Brown Norway rats were exposed by intratracheal instillation of saline, carbon black (CB), or diesel exhaust particles (DEP) (5 mg/kg) on day 1, followed by exposure to ovalbumin (OVA, 90 mg/m(3)) or saline for 30 minutes on days 1, 8, 15, and 29. Animals were sacrificed on day 30. The DEP, CB, or OVA exposure alone did not result in abnormal levels of inflammatory cells, lactate dehydrogenase (LDH), or total protein in the lavage fluid. In combined OVA-DEP or OVA-CB exposure, however, these markers were significantly increased. The adjuvant effect of CB and DEP on OVA sensitization was evidenced by the marked increases in serum OVA-specific IgG (5.6-fold) and IgE (3.5-4 fold) levels, and the increase in interleukin-4 (IL-4) mRNA levels in lung tissue. The OVA exposure markedly reduced glutathione (GSH) levels in both cell types. In combined DEP-OVA exposure, the level of GSH in lymphocytes was further decreased, indicating a possible interactive effect between DEP and OVA exposures. These results show that both DEP and CB augmented OVA-induced allergic sensitization, and that particle composition of DEP may not be a critical factor for the adjuvant effect. OVA exposure causes significant depletion of intracellular GSH in lymphocytes, which may play a key role in OVA-mediated immune responses.
Keywords
Diesel-emissions; Diesel-exhausts; Pulmonary-system-disorders; Lung-disorders; Lung-irritants; Laboratory-animals; Particulates; Respiratory-system-disorders
Contact
HELD, National Institute for Occupational Safety and Health, Morgantown, West Virginia
CODEN
EXLRDA
CAS No.
1333-86-4
Publication Date
20020701
Document Type
Journal Article
Email Address
jma@wvu.edu
Fiscal Year
2002
NTIS Accession No.
NTIS Price
Issue of Publication
5
ISSN
0190-2148
NIOSH Division
HELD
Priority Area
Work Environment and Workforce: Mixed Exposures
Source Name
Experimental Lung Research
State
WV
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