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Effect of sampling time and air humidity on the bioefficiency of filter samples for bioaerosol collection.

Authors
Wang-Z; Reponen-T; Grinshpun-SA; Gorny-RL; Willeke-K
Source
J Aerosol Sci 2001 May; 32(5):661-674
NIOSHTIC No.
20022169
Abstract
The effect of sampling time on the biological collection efficiency of viable airborne microorganisms was studied with two personal filter samplers (the Button Inhalable Aerosol Sampler and the 37-mm cassette) under relative humidities (RH) of 30 and 85%. Polycarbonate filters mounted in the samplers were challenged with five aerosolized microorganisms (fungal spores, endospores, and bacterial vegetative cells) and air was drawn through the samplers for a period of time ranging from 2 min to 8 h. The microorganisms were extracted from the filters at extraction efficiency of 96-98% by vortexing and ultrasonic agitation. The suspension of extracted microorganisms was analyzed by cultivation and by epifluorescence microscopic counting. Most of the tests did not indicate statistically significant differences in the bioefficiency of the two samplers. The culturability of Penicillium melinii and Aspergillus versicolor fungal spores at low and high RH values of 30 and 85% decreased during the first few minutes, but remained approximately the same for sampling times ranging from 30 min to 8 h. The relative culturability of Bacillus subtilis endospores decreased with sampling time, and was 17% at RH=30% and 32% at RH=85%. Pseudomonas fluorescens and Serratia marcescens vegetative cells were culturable only if sampled for 10 min or less. This concluded that the bioefficiency of filter samplers not only depends on the microbial species, but also on the sampling time and relative humidity.
Keywords
Aerosols; Filtration; Microorganisms; Sampling; Airborne-particles; Humidity; Biological-material; Samplers; Filter-materials; Filters; Analytical-instruments; Analytical-processes; Ultrasonic-testing; Equipment-reliability; Author Keywords: Bioaerosols; Filtration; Microorganisms; Spores
Contact
Tiina Reponen, Aerosol Research and Exposure Assessment Laboratory, Department of Environmental Health, University of Cincinnati, Cincinnati, OH 45267-0056, USA
CODEN
JALSB7
Publication Date
20010501
Document Type
Journal Article
Email Address
tiina.reponen@uc.edu
Funding Amount
639953
Funding Type
Grant
Fiscal Year
2001
NTIS Accession No.
NTIS Price
Identifying No.
Grant-Number-R01-OH-003328
Issue of Publication
5
ISSN
0021-8502
Priority Area
Research Tools and Approaches: Exposure Assessment Methods
Source Name
Journal of Aerosol Science
State
OH
Performing Organization
University of Cincinnati, Cincinnati, Ohio
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