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Performance of Air-O-Cell, Burkard, and Button samplers for total enumeration of airborne spores.

Authors
Aizenberg-V; Reponen-T; Grinshpun-SA; Willeke-K
Source
Am Ind Hyg Assoc J 2000 Nov/Dec; 61(6):855-864
NIOSHTIC No.
20022168
Abstract
Performance of three devices used for the total enumeration of airborne spores-the Air-O-Cell sampling cassette, the Burkard personal volumetric air sampler, and the Button Aerosol Sampler--was evaluated under controlled laboratory conditions. The first two are glass-slide impactors; the third collects spores on a filter. The samplers were challenged with 0.44-5.10 microm polystyrene latex particles and five microorganisms of 0.84-3.07 microm mean aerodynamic diameter: Streptomyces albus, Bacillus subtilis, Cladosporium cladosporioides, Penicillium brevicompactum, and Penicillium melinii. An optical particle counter measured the particle concentrations upstream and downstream of each sampler, and thus determined the physical collection efficiency of the three samplers. Collection efficiency of the Button Aerosol Sampler was close to 100% for the entire particle size range studied. The cut-off size of each impactor was 2.3-2.4 microm. Acridine orange (with epifluorescent microscopy) and lactophenol cotton blue (with bright light microscopy) staining techniques were used for the microscopic enumeration of spores. No significant difference in microscopic counts was found (at the 95% significance level) when using these two techniques with the Button Aerosol Sampler filters. When the lactophenol cotton blue staining was used to compare total microbial counts yielded by all three samplers, the Button Sampler showed significantly higher counts for the smaller size microorganisms (B. subtilis and C. cladosporioides). For the larger microorganisms (P. brevicompactum and P. melinii) all three samplers yielded similar results. Uniformity of particle deposition on the collection surface was highest for the Button Aerosol Sampler due to the design of its inlet. Thus, the filter collection method used with the Button Aerosol Sampler is suitable and can be advantageous for the enumeration of total airborne spores.
Keywords
Aerosol-sampling; Filtration; Airborne-particles; Laboratory-work; Exposure-levels
Contact
S.A. Grinshpun, Aerosol Research and Exposure Assessment Laboratory, Department of Environmental Health, University of Cincinnati, Cincinnati, OH 45267-0056
CODEN
AIHAAP
Publication Date
20001101
Document Type
Journal Article
Funding Amount
639953
Funding Type
Grant
Fiscal Year
2001
NTIS Accession No.
NTIS Price
Identifying No.
Grant-Number-R01-OH-003328; Grant-Number-T42-CCT-510420
Issue of Publication
6
ISSN
0002-8894
Priority Area
Research Tools and Approaches: Exposure Assessment Methods
Source Name
American Industrial Hygiene Association Journal
State
OH
Performing Organization
University of Cincinnati, Cincinnati, Ohio
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