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Differential role of hydrogen peroxide in UV-induced signal transduction.

Authors
Ding-M; Li-J; Leonard-SS; Shi-X; Costa-M; Castranova-V; Vallyathan-V; Huang-C
Source
Mol Cell Biochem 2002 May-Jun; 234-235(1-2):81-90
NIOSHTIC No.
20022163
Abstract
The present study investigated the differential requirement of ROS in UV-induced activation of these pathways. Exposure of the mouse epidermal C141 cells to UV radiation led to generation of ROS as measured by electron spin resonance (ESR) and by H2O2 and O2. fluorescence staining assay. Treatment of cells with UV radiation or H2O2 also markedly activated Erks, JNKs, p38 kinase and led to increases in phosphorylation of Akt and p70(S6k) in mouse epidermal JB6 cells. The scavenging of UV-generated H2O2 by N-acety-L-cyteine (NAC, a general antioxidant) or catalase (a specific H2O2 inhibitor) inhibited UV-induced activation of JNKs, p38 kinase, Akt and p70(S6k), while it did not show any inhibitory effects on Erks activation. Further, pretreatment of cells with sodium formate (an .OH radical scavenger) or superoxide dismutase (O2-. radical scavenger) did not inhibit any of these pathways. These results demonstrate that H2O2 generation is required for UV-induced phosphorylation of Akt and p70(S6k), and involved in activation of JNKs and p38 kinase, but not Erks
Keywords
Ultraviolet-radiation; Antioxidants; Cancer; Cell-alteration; Cell-biology; Cell-differentiation; Skin-cancer; Oxidative-phosphorylation; Protein-biochemistry; Animal-studies; Molecular-biology; Oxidative-processes; Radiation-effects; Genes; Free-radicals
Contact
Nelson Institute of Environmental Medicine, New York University School of Medicine, 57 Old Forge Road, Tuxedo, NY 10987, USA
CODEN
MCBIB8
Publication Date
20020501
Document Type
Journal Article
Email Address
chuanshu@env.med.nyu.edu
Fiscal Year
2002
NTIS Accession No.
NTIS Price
Issue of Publication
1-2
ISSN
0300-8177
NIOSH Division
HELD
Source Name
Molecular and Cellular Biochemistry
State
WV
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