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Ubiquitin-based sperm assay for the diagnosis of male factor infertility.

Authors
Sutovsky-P; Terada-Y; Schatten-G
Source
Hum Reprod 2001 Feb; 16(2):250-258
NIOSHTIC No.
20021888
Abstract
Sperm morphology is correlated with fertility in men, yet the existing, subjective sperm morphology assays provide only a limited insight into patients' infertility. Here, we provide the experimental background for a new, objective and automated semen assay, based on the cross-reactivity of defective human spermatozoa with antibodies against a proteolytic marker peptide, ubiquitin. Using immunofluorescence and flow cytometry, we screened the spermatozoa from 17 infertility patients and two fertile donors for their cross-reactivity with anti-ubiquitin antibodies. Thirteen out of 17 patients, but neither of two fertile donors, displayed an increased binding of anti-ubiquitin antibodies to sperm surface, that reflected the occurrence of abnormalities in these samples and was corroborated by available clinical data. Highest correlation coefficient (r = 0.432) was obtained with the cleavage rate after IVF. The contribution of male factor was revealed in several couples previously diagnosed with idiopathic infertility. Ubiquitin-cross-reactive sperm-surface proteins thus seem to be a universal marker of semen abnormalities, including sperm head and tail defects and semen contaminants such as spermatids, leukocytes and cellular debris. We propose that the sperm-ubiquitin tag immunoassay (SUTI) may be a valuable diagnostic tool in treatment of male factor and idiopathic infertility.
Keywords
Reproductive-system-disorders; Spermatogenesis; Humans; Reproductive-hazards; Reproduction; Reproductive-effects; Men
CODEN
HUREEE
Publication Date
20010201
Document Type
Journal Article
Funding Amount
235845
Funding Type
Grant
Fiscal Year
2001
NTIS Accession No.
NTIS Price
Identifying No.
Grant-Number-R21-OH-007324
Issue of Publication
2
ISSN
0268-1161
Source Name
Human Reproduction
State
OR; MO
Performing Organization
University of Missouri, Columbia, Missouri
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