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Chronic smoking enhances tachykinin synthesis and airway responsiveness in guinea pigs.

Authors
Kwong-K; Wu-ZX; Kashon-ML; Krajnak-KM; Wise-PM; Lee-LY
Source
Am J Respir Cell Mol Biol 2001 Sep; 25(3):299-305
NIOSHTIC No.
20021783
Abstract
This study tests the hypothesis that the bronchial hyperreactivity induced by chronic cigarette smoke (CS) exposure involves the increased expression and release of tachykinins and calcitonin gene-related peptide (CGRP) from afferent nerve fibers innervating the airways. In guinea pigs chronically exposed to CS (20 min twice daily for 14-17 d), peak response in total lung resistance to capsaicin (1.68 microg/kg, intravenously) was significantly greater than that evoked by the same dose of capsaicin in control (air-exposed) animals. This augmented response in CS-exposed animals was abolished after treatment with CP-99994 and SR-48968, the neurokinin (NK)-1 and NK-2 receptor antagonists, suggesting the involvement of tachykinins in chronic CS-induced airway hyperresponsiveness (AHR). Further, substance P (SP)-like immunoreactivity (LI) and CGRP-LI in the airway tissue were significantly greater in the CS animals than in the control animals. Finally, beta-preprotachykinin (PPT, a splice variant from the PPT A gene encoding tachykinins including SP and NKA) messenger RNA levels as measured by in situ hybridization histochemistry displayed a significant increase in jugular ganglion neurons but not in dorsal root or nodose ganglion neurons. These data suggest that chronic CS-induced AHR is related to an increase in SP synthesis and release in jugular ganglion neurons innervating the lungs and airways.
Keywords
Lung-irritants; Smoking; Laboratory-animals; Animals; Animal-studies; Bronchial-cancer; Cigarette-smoking; Airway-obstruction; Airway-resistance; Exposure-levels; Chronic-exposure; Lung-disease; Lung-disorders; Pulmonary-system-disorders; Respiratory-system-disorders
CODEN
AJRBEL
Publication Date
20010901
Document Type
Journal Article
Fiscal Year
2001
NTIS Accession No.
NTIS Price
Issue of Publication
3
ISSN
1044-1549
NIOSH Division
HELD
Source Name
American Journal Respiratory Cell and Molecular Biology
State
WV; KY
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