Skip directly to search Skip directly to A to Z list Skip directly to page options Skip directly to site content

NIOSHTIC-2 Publications Search

Search Results

Nitric oxide and proinflammatory cytokines in the bronchoalveolar lavage fluid of rats after intratracheal instillation of toxic Stachybotrys chartarum spores.

Authors
Rao-CY; Hirvonen-MR; Burge-HA; Brain-JD
Source
Am J Respir Crit Care Med 2001 Apr; 163(5)(2)(Suppl):A177
NIOSHTIC No.
20021417
Abstract
Rationale: There has been increasing concern regarding inhalation exposure to the fungus Stachybotrys chartarum. The fungus can produce potent mycotoxins that are thought to be the causative agents. We investigated the mechanisms of pulmonary injury and the role of mycotoxins using a rat model. Methods: Rats were intratracheally instilled one time with unaltered S. chartarum spores suspended in saline at 4 spore concentrations (2x10^6 to 2x10^7 spores/ml). The experiments were duplicated with spores that had been extracted in methanol to reduce the mycotoxin content. One day after instillation, the rats were sacrificed and the lungs lavaged. In a third experiment, rats were instilled with 2x10^7 spores/ml and a lavage was performed at 0, 6, 24 and 72 hours post-instillation. Nitric oxide (NO), interleukin-6 (IL-6) and tumor necrosis factor alpha (TNF-a) were measured in the lavage fluid. Results: At 24 hours, there was no change in IL-6 levels at any dose with the unaltered or methanol-extracted spores. TNF-a increased while NO levels decreased in a dose-dependent manner with both unaltered and methanol-extracted spores. For the unaltered spores, NO levels peaked at 6 hours (1.3 +/- 0.5 uM); TNF-a levels at 24 hours (142 +/- 44 pg/ml); IL-6 levels at 72 hours (123 +/- 99 pg/ml). Conclusions: In this animal model, mycotoxins playa dose-dependent role in pulmonary inflammation and injury. The responses from instillation of methanol­ extracted spores indicated that there may be components other than mycotoxins, integral to S. chartarum, that may also induce pulmonary effects.
Keywords
Fungal-diseases; Fungi; Respiratory-irritants; Respiratory-system-disorders; Pulmonary-system-disorders; Laboratory-animals; Animal-studies; Lung-irritants; Lung-disease
CODEN
AJCMED
Publication Date
20010401
Document Type
Abstract
Fiscal Year
2001
NTIS Accession No.
NTIS Price
Issue of Publication
5
ISSN
1073-449X
NIOSH Division
DRDS
Source Name
American Journal of Respiratory and Critical Care Medicine. Abstracts of the American Thoracic Society 2001 International Conference, May 18-23, 2001, San Francisco, California
State
WV; MA
TOP