Effect of vibration on IL-8 production by dermal microvascular endothelial cells.
Lindsley-WG; Johnson-CM; Cezeaux-JL; Kashon-ML
Ann Biomed Eng 2001 Oct; 29(Suppl 1):S-41
Hand-arm vibration syndrome (HAVS) is an occupational illness that occurs in workers who use vibrating hand tools. Symptoms of HAVS include severe vasoconstriction in the fingers in response to cold (also called Raynaud's phenomenon of occupational origin or vibraiton white finger), paresthesia and numbness in the fingers, and a loss of grip strength. Some evidence suggests that repeated vibration exposure results in a chronic inflammatory response, which may be responsible for neurological and vascular tissue damage. The purpose of this study is to use a cell culture model to investigate the effects of vibration on vascular cells. We grew human dermal microvascular endothelial cells on gelatin-coated 35 mm culture dishes and exposed them to mechanical vibration using an electromagnetic shaker. After 24 hours, supernatants were collected from vibrated cells and from stationary controls. Interleukin-8 (IL-8) protein levels in the supernatant were measured using an enzyme immunoassay. Preliminary results indicate that IL-8 release from vibrated cells was greater than from non-vibrated controls. Our results suggest that vibration stimulates the release of inflammatory mediators from microvascular endothelial cells and that this may be involved in the etiology of HAVS.
Vibration; Occupational-health; Exposure-levels; Neurological-reactions; Proteins; Vibration-exposure; Vibration-effects; Hand-tools; Hand-injuries; Workers; Cell-cultures; Models; Etiology
Annals of Biomedical Engineering