Clinical biological monitoring using immunobiochemical methods.
Striley-CAF; Biagini-RE; Snawder-JE; MacKenzie-BA; Hines-CJ
Abstr Pap - Am Chem Soc 2000 Mar; 219:AGRO-U79
Monitoring of urinary metabolites from pesticide applicators' has been routinely used in NIOSH field studies. Commercially available enzyme linked immunosorbant assays (ELISAs) have been successfully used for screening. Currently we are investigating immunochemical biological monitoring methods for organophosphate pesticides including chlorpyrifos. A magneto-particle ELISA for trichloropyridinol (TCP), a human metabolite of chlorpyrifos, was modified for urinary biomonitoring by beta-glucuronidase pretreatment to yield parent TCP. Biological monitoring for exposure to metolachlor necessitated the development of an ELISA for its putative mercapturic acid metabolite. Development of these biological monitoring strategies entails knowledge of agent specific human pharmacokinetics. We have procured a library of human tissue sub-fractions to serve as an in vitro surrogate of human pharmacokinetics. Each tissue sample has been immunochemically and phentotypically characterized for its toxicokinetic potential. Aspects of inter-individual variation in xenobiotic metabolism can then be evaluated as a covariate in a fully integrated system to develop biomonitoring strategies.
Biological-monitoring; Biomarkers; Pesticides; Pesticides-and-agricultural-chemicals; Immunochemistry; Organo-phosphorus-pesticides; Analytical-chemistry; Analytical-processes; Pharmacodynamics
Cynthia A. F. Striley, Division of Biomedical and Behavioral Science, National Institute for Occupational Safety and Health, 4676 Columbia Parkway, Mailstop C-26, Cincinnati, OH 45226
Abstract; Conference/Symposia Proceedings
Abstracts of Papers - American Chemical Society