Skip directly to search Skip directly to A to Z list Skip directly to page options Skip directly to site content

NIOSHTIC-2 Publications Search

Search Results

Effects of asphalt fume condensate (AFC) exposure on the pulmonary cytochrome P450 (CYP450) system.

Authors
Ma-JYC; Yang-HM; Barger-M; Kreich-AJ; Castranova-V
Source
FASEB J 2000 Mar; 14(4):A173
NIOSHTIC No.
20020995
Abstract
Exposure to asphalt fumes is a concern due to the presence of polycyclic aromatic hydrocarbons (PAH) in asphalt. Bioactivation of many PAH requires metabolism by the CYP450 system. The objective of this study was to evaluate the effects of exposure of rats to AFC on the pulmonary CYP450 system. Rats were intratracheally instilled with saline, 0.1, 0.5, or 2 mg AFC for 3 consecutive days and sacrificed the following day. Total microsomal protein and CYP2Bl and CYP1A1 isozyme levels were determined. The activities associated with CYP2B1 and CYP1A1 were measured by the O-dealkylation of 7-pentoxyresorufin (7-PR) and 7-ethoxyresorufin (7-ER), respectively. AFC exposure did not cause significant changes in total microsomal protein content. CYP2B1 and CYP1A1 levels in AFC-exposed lungs were not significantly different from control as determined by Western blot analysis. However, microsomes from AFC-exposed rats exhibited a concentration- dependent increase in the activity of 7-PR and 7-ER O-dealkylase. These results demonstrate that AFC exposure did not alter total microsomal protein or CYP1A1 and CYP2B1 levels in the lung. However, AFC increased CYP2B1 and CYP1A1 activities in lung microsomes, suggesting that AFC exposure may significantly alter metabolism of PAH by the CYP450 system in the lung.
Keywords
Asphalt-fumes; Fumes; Exposure-levels; Polycyclic-aromatic-hydrocarbons; Bioactivation; Laboratory-animals; Animals; Animal-studies; Pulmonary-system-disorders; Respiratory-system-disorders; Lung-disorders
CODEN
FAJOEC
Publication Date
20000315
Document Type
Abstract; Conference/Symposia Proceedings
Fiscal Year
2000
NTIS Accession No.
NTIS Price
Issue of Publication
4
ISSN
0892-6638
NIOSH Division
HELD
Source Name
The FASEB Journal
State
WV; IN
TOP