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Association between tumors developed from beryllium-transformed cells and changes in cancer-related genes.

Authors
Joseph-P; Klishis-ML; Muchnok-TK; Ong-T; Whong-WZ
Source
Environ Mol Mutagen 2000 Jan; 35(Suppl 31):33
NIOSHTIC No.
20020972
Abstract
Beryllium is used extensively in various industrial applications and many workers are potentially exposed to toxic levels of this metal. Even though laboratory studies have documented the carcinogenic potential of beryllium in experimental animals. The underlying molecular mechanisms are poorly understood. In order to elucidate the molecular mechanisms of beryllium-induced tumorigenesis, we have studied the various cancer-related genes that were altered as a result of cellular transformation induced by beryllium. BALB/c-3T3 cells, morphologically transformed with beryllium, were subcutaneously injected into nude mice to develop tumors. DNA and ANA isolated from cell lines derived from the tumors were used to determine the alterations of various cancer-related genes, such as p53, p16, K-ras, c-myc, c-fos. c-jun, c-sis and erbB1. Alterations of the copy number and expression of the genes were studied by differential PCA and ATPCA, respectively. Both the gene copy number and expressions of K-ras, c-fos and c-jun were higher in the tumor cells compared to those of the control Balb/c-3T3 cells. In contrast, p16 exhibited a decrease in both the gene copy number and expression in the tumor cells compared to those of the control cells. These results suggest that beryllium-induced tumorigenesis is associated with alterations of various cancer-related genes, and these alterations may play a role in beryllium-induced tumorigenesis.
Keywords
Tumors; Beryllium-compounds; Cell-transformation; Cancer; Genes; Workers; Occupational-exposure; Metals; Toxic-effects; Laboratory-testing; Laboratory-animals; Animals; Animal-studies; Tumorigens; Tumorigenesis
CODEN
EMMUEG
CAS No.
7440-41-7
Publication Date
20000101
Document Type
Abstract; Conference/Symposia Proceedings
Fiscal Year
2000
NTIS Accession No.
NTIS Price
ISSN
0893-6692
NIOSH Division
HELD
Source Name
Environmental and Molecular Mutagenesis
State
WV
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