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Ovalbumin aeroallergen exposure-response in brown Norway rats.

Authors
Siegel-PD; Al-Humadi-NH; Millecchia-LL; Robinson-VA; Hubbs-AF; Nelson-ER; Fedan-JS
Source
Inhal Toxicol 2000 Mar; 12(3):245-261
NIOSHTIC No.
20020810
Abstract
A major route of exposure to allergens is through the respiratory tract. Comparatively few animal studies have used aerosolized high-molecular-weight allergens for sensitization, and in these studies, proper characterization of the aeroallergen exposure was usually missing. The purpose of this study was to profile the exposure-response relationship in Brown Norway rats (BNR) to well-characterized ovalbumin (OVA) aerosols. Rats were exposed 30 min/wk x 6 wk to respirable OVA aerosols from <1 mg/m(3) to 64 mg/m(3) air. Ovalbumin-specific circulating immunoglobulin (Ig)E, IgG, and IgA were measured throughout the study period. Rats were sacrificed 1 day after the last exposure. Pulmonary tissue was processed for histopathological and histochemical analysis. Tracheas were isolated, perfused, and assessed for in vitro responsiveness to methacholine. Serum concentrations of OVA-specific antibodies increased with both exposure concentration and number of exposures. The number of BNR with measurable titers also increased with both dose and time. Pulmonary inflammatory changes were noted only in BNR exposed to higher OVA concentrations (15 and 64 mg/m(3) air). Increased tracheal reactivity to methacholine was not found in any of the sensitized BNR. In summary, sustained aeroallergen concentration-dependent changes in specific antibody responses and pulmonary inflammation have been demonstrated.
Keywords
Laboratory-animals; Animals; Animal-studies; Exposure-levels; Exposure-assessment; Allergens; Sensitization; Aerosols; Histochemical-analysis; Histopathology; Pulmonary-system-disorders; Respiratory-system-disorders; Antibody-response
Contact
Paul D. Siegel, PhD, Team Leader / Bioorganic Chemistry, Analytical Service Branch, HELD/NIOSH, 1095 Willowdale Road, Morgantown, WV 26505, USA
CODEN
INHTE5
Publication Date
20000301
Document Type
Journal Article
Email Address
pds3@cdc.gov
Fiscal Year
2000
NTIS Accession No.
NTIS Price
Issue of Publication
3
ISSN
0895-8378
NIOSH Division
HELD
Source Name
Inhalation Toxicology
State
WV
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