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Cellular delivery of functional peptides to block cytokine gene expression.

Authors
Rojanasakul-Y; Luo-Q; Ye-J; Antonini-JM; Toledo-D
Source
J Control Release 2000 Mar; 65(1-2):13-17
NIOSHTIC No.
20020795
Abstract
Advances in molecular and cellular biology have identified the cellular mediators that regulate many disease processes and have facilitated the development of new therapeutic agents that control these events. However, the size, complexity, and cellular inaccessibility of these therapeutic agents make their cellular delivery difficult. Here, we describe an efficient cellular delivery system that exploits the membrane-translocating ability of signal peptides to import functional peptides into cells. Molecular conjugates consisting of the signal import peptide (IP) and nuclear localization sequence (NLS) of the transcription factor NF-kappaB were synthesized. Electrophoretic mobility shift and enzyme-linked immunosorbent assays were used to assess the inhibitory effects of these synthetic peptides on agonist-induced NF-kappaB nuclear translocation and transcriptional activation. Our results indicated that the peptides were effective in inhibiting both the nuclear translocation and transcriptional activation of NF-kappaB. However, their effects required the presence of the IP moiety for efficient cellular entry of the NLS. Structural analysis of IP showed that the hydrophobic domain, and to a lesser extent the N-terminal domain, was responsible for the membrane translocating activity of IP.
Keywords
Cellular-function; Peptides; Genes; Molecular-biology; Therapeutic-agents
Contact
Department of Basic Pharmaceutical Sciences, West Virginia University, School of Pharmacy, PO Box 9530, Morgantown, WV 26506, USA
CODEN
JCREEC
Publication Date
20000301
Document Type
Journal Article
Email Address
yrojanasakul@hsc.wvu.edu
Fiscal Year
2000
NTIS Accession No.
NTIS Price
Issue of Publication
1-2
ISSN
0168-3659
NIOSH Division
HELD
Source Name
Journal of Controlled Release
State
WV; PA
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