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Mast cell basic fibroblast growth factor in silicosis.

Authors
Hamada-H; Vallyathan-V; Cool-CD; Barker-E; Inque-Y; Newman-LS
Source
Am J Respir Crit Care Med 2000 Jun; 161(6):2026-2034
NIOSHTIC No.
20020686
Abstract
To investigate the role of mast cells (MC) and their fibrogenic growth factors in silicosis, we performed quantitative immunohistochemistry for MC tryptase and for basic fibroblast growth factor (bFGF) in lung tissue from silicotic and control subjects. Anti-bFGF antibody was bound to lung MC, basement membrane, endothelial cells, and smooth-muscle cells. Morphometric analysis revealed that the volume density (V(v)) of MC was increased in silicotic lung and that the V(v) of bFGF-positive (bFGF(+)) cells was significantly higher than normal in silicotic lung. Most MC contained bFGF (rho = 0.88, p < 0.001). The V(v) of collagen/reticulin fibers was increased in silicosis and correlated with the V(v) of bFGF(+) cells (rho = 0.81, p < 0.001). Immature silicotic nodules contained bFGF(+) MC throughout the loose array of collagen/reticulin fibers. In large, mature nodules, the density of collagen/reticulin fibers was higher, and bFGF(+) MC were found only in the nodule periphery. Because of this circumferential MC alignment in silicotic nodules, we observed a negative correlation between the V(v) of bFGF(+) MC and the density of collagen/reticulin fibers in silicotic nodules (rho = -0.80, p < 0.001) and between the V(v) of all other nodule-associated cells and the density of collagen/reticulin fibers in the hypocellular nodule centers (rho = -0.84, p < 0.001). We conclude that MC that produce bFGF may play an important role in the development of silicosis.
Keywords
Silicosis; Quantitative-analysis; Lung-tissue; Pulmonary-system-disorders; Respiratory-system-disorders; Lung-cells; Lung-fibrosis; Fibrogenicity
Contact
Lee S. Newman, M.D., National Jewish Medical and Research Center, Division of Environmental and Occupational Health Sciences, 1400 Jackson Street, Denver, CO 80206
CODEN
AJCMED
Publication Date
20000601
Document Type
Journal Article
Fiscal Year
2000
NTIS Accession No.
NTIS Price
Issue of Publication
6
ISSN
1073-449X
NIOSH Division
HELD
Source Name
American Journal of Respiratory and Critical Care Medicine
State
WV; CO
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