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Effects of simulated pulmonary surfactant on the cytotoxicity and DNA-damaging activity of respirable quartz and kaolin.

Authors
Gao-N; Keane-MJ; Ong-T; Wallace-WE
Source
J Toxicol Environ Health, A 2000 Jun; 60(3):153-167
NIOSHTIC No.
20020679
Abstract
Respirable-sized quartz and kaolin dusts were pretreated with simulated pulmonary surfactant dispersions of dipalmitoyl phosphatidylcholine (DPPC) in saline to model the conditioning of particles depositing in alveolar regions of the lung. DPPC-treated and untreated dusts were used to challenge lavaged rat pulmonary alveolar macrophages in vitro. Cytotoxicity was determined over a 5-d period using both total and viable cell counts from a fluorescence-based viability assay. DNA damage, as an indication of genotoxicity, was determined over a 7-d period by the single-cell gel electrophoresis assay. Untreated quartz and kaolin both expressed a significant and potent cytotoxicity, which increased with concentration and time. DPPC-surfactant pretreatment delayed significant expression of this cytotoxicity until 3 to 5 d after challenge. Untreated quartz also caused DNA damage, which increased with concentration and time. DPPC-surfactant treatment of quartz delayed most DNA damage expression to 5 and 7 d. Untreated kaolin expressed weaker activity for DNA damage, significant at the highest concentration through 5 d, and at the higher concentrations on d 7. Surfactant treatment delayed most kaolin activity for DNA damage to 7 d after challenge.
Keywords
Simulation-methods; Cytotoxicity; Quartz-dust; Respirable-dust; Models; Laboratory-animals; Animals; Animal-studies; Lung-disorders; Pulmonary-system-disorders; Respiratory-system-disorders; In-vitro-studies
Contact
William E. Wallace, NIOSH, 1095 Willowdale Road, Morgantown, WV 26505, USA
CODEN
JTEHD6
CAS No.
14808-60-7; 1332-58-7
Publication Date
20000609
Document Type
Journal Article
Email Address
wew2@cdc.gov
Fiscal Year
2000
NTIS Accession No.
NTIS Price
Issue of Publication
3
ISSN
1528-7394
NIOSH Division
HELD
Source Name
Journal of Toxicology and Environmental Health, Part A: Current Issues
State
WV
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