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Involvement of 5'-flanking kB-like sites within bcl-x gene in silica-induced bcl-x expression.

Authors
Chen-F; Demers-LM; Vallyathan-V; Castranova-V; Shi-X
Source
J Biol Chem 1999 Dec; 274(50):35591-35595
NIOSHTIC No.
20000958
Abstract
The present study investigated the involvement of the transcription factor NF-kappaB in the expression of an anti-apoptotic gene, bcl-x, using a murine macrophage cell line and peritoneal macrophages from both wild type p50(+/+)) and NF-kappaB p50 gene knockout (p50(-/-)) mice. Increased expression of Bcl-x protein was observed in native and silica-exposed p50(-/-) macrophages in which the NF-kappaB-containing complex was predominantly induced. Co-transfection experiment using a bcl-x promoter reporter construct and an expression vector for NF-kappaB p50 or p65 indicates that p65, but not p50, up-regulates the promoter activity of the bcl-x gene. DNA sequence analysis revealed that there are several kappaB-like sites within the 5'-flanking region of the bcl-x gene. Electrophoretic mobility shift assay suggested differences in binding of the NF-kappaB complexes to these putative NF-kappaB binding sites of the bcl-x gene.
Keywords
Genetics; Carcinomas; DNA-damage; Cellular-reactions; In-vitro-studies
Contact
Pathology and Research Branch NIOSH 1095 Willowdale Rd. Morgantown, WV 26505
CODEN
JBCHA3
Publication Date
19991210
Document Type
Journal Article
Fiscal Year
2000
NTIS Accession No.
NTIS Price
Issue of Publication
50
ISSN
0021-9258
NIOSH Division
HELD
Priority Area
Other Occupational Concerns
Source Name
Journal of Biological Chemistry
State
WV; PA
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