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Collection of airborne microorganisms by electrostatic precipitation.

Authors
Mainelis-G; Grinshpun-SA; Willeke-K; Reponen-T; Ulevicius-V; Hintz-PJ
Source
Aerosol Sci Tech 1999 Mar; 30(2):127-144
NIOSHTIC No.
20000903
Abstract
The applicability of electrostatic precipitation as a method for bioaerosol collection was investigated by using a modified Electrostatic Aerosol Sampler (EAS) (Model 3100, TSI Inc., St. Paul, MN). The physical and biological efficiencies of this method were determined. THe tests were performed using three bacterial species which were collected onto agar, into water and onto filters. The physical collection efficiency was higher than 80% when using a sampling flow rate of 1 L/min. When the Bacillus subtilis var niger (BG) spores were collected on agar, about 50-60% of the collected culturable organisms formed colonies. The bioefficiency exceeded 90% when the BG spores were collected on a filter, but was only 15-22% when collected into water. The Mycobacterium bovis BCG bacteria recovered at the 0-8% level on all three collection media. The least number of colonies were formed when Pseudomonas fluorescens bacteria were collected on any of the collection media. This data shows that the process of electrostatic collection is very complex for sensitive airborne bacteria and thus several effects should be considered when assessing its bioefficiency. In separate tests conducted without aerosol flow through the sampler, bacteria placed on the collection media did not show any significant reduction in bacterial recovery while exposed to a strong electric field. It was found that evaporation from the collection media, such as agar of water, increases the humidity inside the EAS and may affect the size distribution of the particles being collected, resulting in decreased physical and biological efficiencies of the electrostatic precipitation method, For hardy microorganisms such as BG spores, the bioefficiency for electrostatic collection is high, thus encouraging further explorations of the electrostatic method for sampling bioaerosols.
Keywords
Microorganisms; Air-contamination; Airborne-particles; Electrostatic-filters; Sampling; Aerosols
Contact
Aerosol Research and Exposure Assessment Laboratory, Department of Environmental Health, University of Cincinnati, P.O. Box 670056, Cincinnati, OH 45267-0056
CODEN
ASTYDQ
Publication Date
19990301
Document Type
Journal Article
Fiscal Year
1999
NTIS Accession No.
NTIS Price
Issue of Publication
2
ISSN
0278-6826
NIOSH Division
DRDS
Source Name
Aerosol Science and Technology
State
WV
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