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Effect of fiber length on glass microfiber cytotoxicity.

Authors
Blake-T; Castranova-V; Schwegler-Berry-D; Baron-P; Deye-GJ; Li-C; Jones-W
Source
J Toxicol Environ Health, A 1998 Jun; 54(4):243-259
NIOSHTIC No.
20000648
Abstract
Fiber length has been implicated as a determinant of fiber toxicity. Fibers of narrowly defined length can be generated by dielectrophoretic classifiers. Since the quantities of fibers produced are very small, we developed a rat alveolar macrophage microculture system to study the toxicity of these samples. The objective of this study was to examine the role of fiber length on the cytotoxicity of Manville code 100 (JM-100) fibers. Rat alveolar macrophages were cultured with 0-500 mug/ml of 5 lengths of JM-100 fibers on 96-well plates. After 18 h, well supernatants were removed and lactate dehydrogenase (LDH) activity was measured to assess cell damage. Chemiluminescence (CL), an assessment of macrophage function, was measured by adding lucigenin with or without zymosan, a particulate stimulus, to appropriate wells. For each fiber length the effects were concentration dependent: CL declined and LDH rose with increasing fiber concentration. Comparing the effects of different lengths showed the greatest toxicity from a relatively long fiber sample (mean length = 17 mum). Microscopic examination of the interaction of fibers with macrophages revealed multiple macrophages attached along the length of the long fibers. This suggests that frustrated, or incomplete, phagocytosis may be a factor in the increased toxicity of longer fibers. Overall the results demonstrate that length is an important determinant of toxicity for JM-100 fibers.
Keywords
Cell-death; Glass-fibers; Asbestos; Ceramic-fibers; Fiber-length; Fiber-toxicity;
CODEN
JTEHD6
CAS No.
2315-97-1; 9010-72-4
Publication Date
19980615
Document Type
Journal Article
Fiscal Year
1998
NTIS Accession No.
NTIS Price
Issue of Publication
4
ISSN
0098-4108
NIOSH Division
DRDS
Source Name
Journal of Toxicology and Environmental Health, Part A: Current Issues
State
WV
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