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Deferoxamine inhibition of Cr(V)-mediated radical generation and deoxyguanine hydroxylation: ESR and HPLC evidence.

Authors
Shi-X; Sun-X; Gannett-PMN; Dalal-NS
Source
Arch Biochem Biophys 1992 Mar; 293(2):281-286
NIOSHTIC No.
10012238
Abstract
Electron spin resonance (ESR) and high-performance liquid chromatography (HPLC) techniques were utilized to investigate the effect of deferoxamine on free radical generation in the reaction of Cr(V) with H2O2 and organic hydroperoxides. ESR measurements demonstrated that deferoxamine can efficiently reduce the concentration of the Cr(V) intermediate as formed in the reduction of Cr(VI) by NAD(P)H or a flavoenzyme glutathione reductase/NADH. ESR spin trapping studies showed that deferoxamine also inhibits Cr(V)-mediated ·OH radical generation from H2O2, as well as Cr(V)-mediated alkyl and alkoxy radical formation from t-butyl hydroperoxide and cumene hydroperoxide. HPLC measurements showed that ·OH radicals generated by the Cr(VI)/flavoenzyme/ NAD(P)H enzymatic system react with 2'-deoxyguanine to form 8-hydroxy-2'-deoxyguanine (8-OHdG), a DNA damage marker. Deferoxamine effectly inhibited the formation of 8-OHdG also.
Keywords
Liquid-chromatography; Free-radical-generation; Free-radicals; Chromium-compounds; Chromatographic-analysis; DNA-damage
CODEN
ABBIA4
CAS No.
7440-47-3; 18540-29-9
Publication Date
19920301
Document Type
Journal Article
Funding Type
Cooperative Agreement
Fiscal Year
1992
NTIS Accession No.
NTIS Price
Identifying No.
MIR 28-93; Cooperative-Agreement-Number-U60-CCU-306149
Issue of Publication
2
ISSN
0003-9861
Source Name
Archives of Biochemistry and Biophysics
State
DC; WV
Performing Organization
Center to Protect Workers' Rights
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