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Development of a bioassay for pulmonary cell production of fibrogenic factors.

Authors
Reist-R; Bryner-K; Wearden-P; Blackford-J; Vrana-K; Castranova-V; Dey-R
Source
Toxicology Methods 1991 Jan; 1(1):53-65
NIOSHTIC No.
10012236
Abstract
Fibroblast proliferation and enhancement of collagen synthesis are key steps in the development and progression of pulmonary fibrosis. The current investigation presents a fibroblast proliferation assay to detect the release of competence factors from pneumocytes. The specific objective of this study was to define cell culture conditions for fibroblasts that would allow selective detection of competence factors in test media. The results indicate the following: (a) fibroblasts should be cultured for 2 days in plasma-free medium rather than 2% plasma to assure complete quiescence; (b) 1% plasma was optimum for producing progression activity for the bioassay of a competence factor, while 0.5% plasma did not produce sufficient progression activity (yielding false negatives) and 2% plasma often contained significant competence activity (yielding false positives); and (c) fresh plasma should be used since the progression activity of plasma was adversely affected by freezing and thawing. Under these conditions, this bioassay can be employed with either rat or human lung fibroblasts to detect competence factors, such as platelet-derived growth factor (PDGF), at levels as low as 25 ng/ml.
Keywords
Bioassays; Reproductive-system-disorders; Pulmonary-system-disorders; Fibrosis; Fibrogenesis; Fibrogenicity
CODEN
TOMEEB
Publication Date
19910101
Document Type
Journal Article
Fiscal Year
1991
NTIS Accession No.
NTIS Price
Identifying No.
MIR 30-93; Grant-Number-5431
Issue of Publication
1
ISSN
1051-7235
NIOSH Division
DRDS
Source Name
Toxicology Methods
State
WV
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