The purpose of this study was to examine effects of acid mine drainage (AMD) originating from volcanic, igneous, and sedimentary watersheds in the Colorado mineral belt on stream chemistry and macroinvertebrates. Criteria for the selection of study watersheds were a well-defined point source of AMD, presence of suitable control site, and accessibility. The selected watersheds were Kerber Creek near Villa Grove in the San Luis Valley, Peru Creek near Dillon in central Colorado, and Coal Creek near Crested Butte. The report presents results of monthly sampling conducted from July- September 1980 through May-July 1981. Kerber and Peru Creeks received AMD throughout the study period. In June 1981, a treatment plant began operations that significantly improved water quality in Coal Creek, thus allowing an assessment of reclamation success.