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Protein damage caused by occupational toxicants.

Authors
Keller-R
Source
Division of Toxicology, University of Arkansas for Medical Sciences, University of Arkansas, Little Rock, Arkansas 1997 Jun; :1-9
Link
NIOSHTIC No.
00241071
Abstract
Studies were conducted to determine whether certain occupational toxicants are capable of causing tissue damage by oxidatively modifying proteins and that these oxidized proteins can be detected using polyclonal antibody techniques to provide a measure of exposure assessment. To investigate this theory, an immunochemical assay was developed to detect oxidative protein damage using a polyclonal antibody assay and validated by direct comparison with the established spectrophotometric techniques. The utility of the immunoassay was then tested to detect oxidized proteins in animals exposed to oxidants found in the occupational environments. The findings indicated that the technique is valid for immunochemical determination of protein oxidation. The technique could be used to determine carbonyl groups formed by oxidation of protein by either metal catalyzed or radiolytically generated oxygen radicals. A comparison of the technique with the spectrophotometric assay indicates that oxidative damage can be detected immunochemically using three orders of magnitude less protein. The results further suggest that the technique has limited usefulness for identifying oxidative damage to protein tissue fractions from tissue treated in- vitro or in-vivo, primarily due to the high background of oxidized proteins that occur during normal cellular physiology.
Keywords
NIOSH-Grant; Pulmonary-system-disorders; Oxidative-processes; Respiratory-system-disorders; Lung-irritants; Immunology; Immunological-tests; Biological-monitoring; Antibody-response; Occupational-exposure
Contact
Pharmacology-Toxicology Univ of Arkansas for Medical S 4301 West Markham, Slot 638 Little Rock, AR 72205
Publication Date
19970602
Document Type
Final Grant Report
Funding Amount
64628
Funding Type
Grant
Fiscal Year
1997
NTIS Accession No.
PB98-137771
NTIS Price
A02
Identifying No.
Grant-Number-R03-OH-03061
NIOSH Division
OEP
Priority Area
Pulmonary System Disorders
Source Name
Division of Toxicology, University of Arkansas for Medical Sciences, University of Arkansas, Little Rock, Arkansas
State
AR; KS
Performing Organization
University of Arkansas Med Scis Ltl Rock, Little Rock, Arkansas
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