Skip directly to search Skip directly to A to Z list Skip directly to page options Skip directly to site content

NIOSHTIC-2 Publications Search

Search Results

Application of the gas chromatography-fatty scid methyl ester system for the identification of environmental and clinical isolates of the family Micrococcaceae.

Authors
Pendergrass-SM; Jensen-PA
Source
Appl Occup Environ Hyg 1997 Aug; 12(8):543-546
NIOSHTIC No.
00238932
Abstract
The gas chromatography (GC) fatty acid methyl ester (FAME) microbial identification system (MIS) for characterizing environmental and clinical staphylococci and related organisms was evaluated. Bacteria from the American Type Culture Collection were used in this study. About 40mg of the bacterial colonies underwent saponification to convert their cellular fatty acids to fatty acid methyl esters. Of the 98 cultures obtained for the study, 17% were originally clinical isolates and 83% were environmental isolates. Differences in the FAME profiles of the Staphylococcus and Micrococcus species were sufficient to distinguish between closely related species. Bacteria were cultured, and FAME analytes were prepared. GC analysis of FAME analytes was conducted, using a flame ionization detector. The identification of bacteria by SIs using the MIS was based on the average FAME profiles of about 100 geographically different isolates for each test strain analyzed. Analysis of four old cultures showed that similarity indices (SI) fell as cultures aged. Based on historical analysis, an SI of 0.3 or greater was acceptable. Of the 98 strains, 96 were correctly identified, and another was identified, but with an SI below the 0.3 threshold. The authors conclude that the GC/FAME method of bacterial identification could serve as a primary means for identifying culturable Staphylococcus and Micrococcus species from bulk and air samples collected during indoor environmental quality investigations.
Keywords
NIOSH-Author; Microbiology; Microorganisms; In-vitro-studies; Cell-cultures; Indoor-air-pollution; Chromatographic-analysis; Indoor-environmental-quality
CODEN
AOEHE9
Publication Date
19970801
Document Type
Journal Article
Fiscal Year
1997
NTIS Accession No.
NTIS Price
Issue of Publication
8
ISSN
1047-322X
NIOSH Division
DPSE
Source Name
Applied Occupational and Environmental Hygiene
State
OH
TOP