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Asbestos induced alteration in endothelial cell function.

Authors
Treadwell-MD
Source
The Environmental Affairs Group, Sanders A. Lockheed Martin Company, Nashua, New Hampshire 1997 Jan; :1-6
Link
NIOSHTIC No.
00238184
Abstract
The purpose of this research was to investigate whether asbestos (1332214) induces an active endothelial cell phenotype, resulting in the increased expression of growth factors and proteases, which are relevant to the development of fibrosis. Porcine aortic endothelial cells were cultured with asbestos and ceramic fibers. The findings indicated that endothelial cells respond to noncytotoxic concentrations of asbestos fibers with alterations in specific cell functions, which may be relevant to pulmonary disease processes. The cells respond to both chrysotile (12001295) and crocidolite (12001284) asbestos fibers by changing cell morphology in focal areas of fiber contact, assuming a spindled appearance, indicative of the activated endothelial cell. A time dependent increase was noted in neutrophil binding to Intercellular-Adhesion-Molecule-1 and increases in both Urokinase-like-Plasminogen-Activator (uPA) mRNA levels and uPA activity. Endothelial cells exposed to chrysotile asbestos demonstrated in a time dependent manner an increase in the expression of the cell surface uPA receptor. Exposed cells also demonstrated an increase in the activation of the transcription activating factor Nuclear-Factor-kappa-B (NF-kB). Refractory-Ceramic- Fiber-Type-1 did not stimulate the endothelial cell morphology alteration, increase PMN binding, uPA mRNA or activity, or increase NF-kB nuclear translocation.
Keywords
NIOSH-Grant; Pulmonary-system-disorders; Asbestos-fibers; Fibrous-dusts; Lung-irritants; Respiratory-system-disorders; Cytotoxic-effects; Cell-cultures; Mammalian-cells
Contact
Pharmacology and Toxicology Dartmouth Medical School Remsen 7650 Hanover, NH 03755-3835
CAS No.
1332-21-4; 12001-29-5; 12001-28-4
Publication Date
19970114
Document Type
Final Grant Report
Funding Amount
76183
Funding Type
Grant
Fiscal Year
1997
NTIS Accession No.
PB97-206296
NTIS Price
A02
Identifying No.
Grant-Number-R03-OH-03267
NIOSH Division
OEP
Priority Area
Pulmonary-system-disorders; Respiratory-system-disorders
Source Name
The Environmental Affairs Group, Sanders A. Lockheed Martin Company, Nashua, New Hampshire
State
NH
Performing Organization
Dartmouth College, Hanover, New Hampshire
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