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Characterization of histamine releasing factors in diisocyanate-induced occupational asthma.

Authors
Lummus-ZL; Alam-R; Bernstein-JA; Bernstein-DI
Source
Toxicology 1996 Jul; 111(1-3):191-206
NIOSHTIC No.
00234232
Abstract
Antigen specific histamine releasing factors (HRF), associated with diisocyanate (DI) induced asthma, were studied in 32 workers exposed to DI. All workers presented with occupational asthma (OA) associated with DI exposure. Venous blood was collected for characterization of peripheral blood mononuclear cells (PBMC), HRF, monocyte chemoattractant proteins (MCP), and the chemokine known as regulated on activation normal-T expressed and secreted (RANTES). Mean values for HRF in OA workers were greater than for asymptomatic exposed workers or unexposed controls after stimulation of PBMC with DI antigens. HRF was significantly associated with clinical diagnosis of OA. Specific immunoglobulin-E or immunoglobin-G antibody production did not correlate with OA. Hapten specific enhancement of HRF was significantly greater when PBMCs were stimulated in-vitro with the DI chemicals to which workers had been exposed. Immunoglobin-G antibodies, but not immunoglobulin-E, also demonstrated humoral immune response to DI antigens. Symptomatic workers showed higher mean production of antigen stimulated MCP and HRF than asymptomatic controls, but RANTES production was similar in both groups. A greater synthesis of antigen stimulated MCP messenger RNA occurred in an asthmatic worker than in a normal control. Purified T-cells from asymptomatic workers showed normal CD4/CD8 ratios (2.2), whereas low CD4/CD8 ratios (1.0 to 1.4) occurred in OA workers. The study confirmed that PBMC of workers with known OA induced by DI can be stimulated in-vitro to produce basophil activating HRF and MCP. The authors conclude that the HRF bioassay appears to detect a cell mediated immune response to DI haptens in workers with DI induced OA.
Keywords
NIOSH-Publication; NIOSH-Grant; Pulmonary-system-disorders; Occupational-exposure; Occupational-hazards; Occupational-diseases; Occupational-health; Humans; Bronchial-asthma; Airway-resistance; Respiratory-irritants
Contact
Internal Medicine University of Cincinnati Med C 231 Bethesda Ave. Cincinnati, Ohio 45267-0563
CODEN
TXCYAC
Publication Date
19960717
Document Type
Journal Article
Funding Amount
97200
Funding Type
Grant
Fiscal Year
1996
NTIS Accession No.
NTIS Price
Identifying No.
Grant-Number-K01-OH-00073
Issue of Publication
1-3
ISSN
0300-483X
Priority Area
Pulmonary-system-disorders
Source Name
Toxicology
State
OH
Performing Organization
University of Cincinnati, Cincinnati, Ohio
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