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Generation of reactive oxygen species by quartz particles and its implication for cellular damage.

Authors
Shi-X; Mao-Y; Daniel-LN; Saffiotti-U; Dalal-NS; Vallyathan-V
Source
Appl Occup Environ Hyg 1995 Dec; 10(12):1138-1144
NIOSHTIC No.
00232493
Abstract
The oxygen species generating potential and genotoxic effects of freshly fractured quartz (14808607) were examined. Wet analytical chemistry and electron spin resonance trapping analyses demonstrated the generation of hydrogen-peroxide (7722841), hydroxyl radicals, superoxide radicals, and singlet oxygen (7782447) by freshly fractured quartz particles. The degree of hydroxyl radical generation was inhibited by superoxide-dismutase, catalase, and deferoxamine, and enhanced by hydrogen-peroxide. The freshly fractured quartz was also found to consume molecular oxygen and induce lipid peroxidation in a dose dependent fashion. Lipid peroxidation was inhibited by superoxide-dismutase, catalase, and sodium-benzoate (532321). Freshly fractured silica particles induced breaks when incubated with double stranded DNA. This result did not occur when the incubation was conducted in the absence of oxygen or in the presence of catalase. The authors conclude that freshly fractured quartz particles appear to generate reactive oxygen species that may play a role in quartz induced cellular injury.
Keywords
NIOSH-Author; NIOSH-Cooperative-Agreement; Quartz-dust; Silica-dusts; Free-radicals; Oxidative-processes; Genotoxic-effects; Pathogenesis
CODEN
AOEHE9
CAS No.
14808-60-7; 7722-84-1; 7782-44-7; 532-32-1
Publication Date
19951201
Document Type
Journal Article
Funding Amount
2937066
Funding Type
Cooperative Agreement
Fiscal Year
1996
NTIS Accession No.
NTIS Price
Identifying No.
Cooperative-Agreement-Number-U60-CCU-306149
Issue of Publication
12
ISSN
1047-322X
Priority Area
Pulmonary-system-disorders
Source Name
Applied Occupational and Environmental Hygiene
State
DC
Performing Organization
Center to Protect Workers' Rights
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