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Primary contribution of the injector to carryover of a trace analyte in high-performance liquid chromatography.

Authors
Saha-M; Giese-RW
Source
J Chromatogr A 1993 Feb; 631(1/2):161-163
NIOSHTIC No.
00232471
Abstract
A study was undertaken to determine the extent to which the injector contributes to the problem of analyte carryover in high performance liquid chromatography (HPLC). Two experiments were conducted to measure a low nanogram amount of N1,N3-bis(pentafluorobenzyl)-N7-(2- (pentafluorobenzyloxy)ethyl)xanthine using HPLC. Despite immediate washing of the injector and HPLC column, analyte carryover was seen after injection of the pure mobile phase. Possible mechanisms for analyte retention in the injector were suspected to be active sites and/or solvent dead volumes. Comparable carryover was seen in trials where gradients were conducted immediately following installation of the clean injector, indicating that the isocratic washing procedure was as effective as gradient conduction. Total carryover in all trials remained below 1% of the originally injected analyte. From this study the authors conclude that the use of two injectors each dedicated to different analyte levels can alleviate the problem of injector induced analyte carryover in HPLC.
Keywords
NIOSH-Grant; NIOSH-Publication; Grants-other; Analytical-processes; Analytical-methods; Trace-analysis; Chromatographic-analysis; Gas-liquid-chromatography; Sample-preparation
Contact
Medicinal Chemistry Northeastern University 360 Huntington Avenue Boston, MA 02115
CODEN
JCRAEY
CAS No.
126565-13-7
Publication Date
19930212
Document Type
Journal Article
Funding Amount
433637
Funding Type
Grant
Fiscal Year
1993
NTIS Accession No.
NTIS Price
Identifying No.
Grant-Number-R01-OH-02792
Issue of Publication
1/2
ISSN
0021-9673
Priority Area
Other Occupational Concerns; Grants-other
Source Name
Journal of Chromatography A
State
ME; MA
Performing Organization
Northeastern University, Boston, Massachusetts
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