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Chlorophyllin: an antigenotoxic agent.

Authors
Ong-T; Brockman-HE; Whong-Z
Source
Food Phytochemicals for Cancer Prevention, I. Fruits and Vegetables. Huang M-T, ed. Washington, DC: American Chemical Society, ACS Symposium Series 546, 1994 Jan; 1:272-281
Link
NIOSHTIC No.
00232439
Abstract
The antimutagenic and antitransforming activities of chlorophyllin were investigated and compared with the antimutagenic activities of vitamin-C vitamin-E, retinol and beta-carotene. In the Ames Salmonella reverse mutation test, a concentration of 1.25 milligrams (mg) chlorophyllin per plate inhibited the mutagenicity of airborne particles (AP), cigarette smoke (CS), fried shredded pork (FSP) and fried beef (FB). Inhibitions of 92% to 98% by chlorophyllin of the mutagenicity of diesel emission particles (DEP), coal dust (CD), red grape juice (RGJ), and red wine (RW) were observed. Inhibitions of 75% to 80% by concentrations of 10mg chlorophyllin per plate were seen in the mutagenicity of tobacco snuff (TS) and chewing tobacco (CT). Concentrations of 2.5mg chlorophyllin per plate achieved inhibitions of 60% to 100% in mutagenicity of AP, CD, DEP, TS, and BP in the forward mutagen assay. Inhibition of DEP mutagenicity was not decreased by heating chlorophyllin for 10 minutes at 100 degrees- C. Reconstruction assays demonstrated that the observed inhibition of mutagenicity of test mixtures by chlorophyllin was not the result of inhibition of revertant cell divisions. At equal concentrations, retinol exhibited 50% of the inhibitory effect of chlorophyllin of the complex mixtures tested. Vitamin-E and beta-carotene inhibited less than 20% of the mutagenicity of the test mixtures, with the exception of AP, which beta-carotene inhibited by 39%. Vitamin-C inhibited none of the mutagenicities, increasing the mutagenic effect of AP by 60%. At a concentration of 150 micrograms/milliliter, chlorophyllin inhibited the transforming activities of benzo(a)pyrene (50328), N-methyl-N'-nitro-N- nitroguanidine (70257), 3-methylcholanthrene (56495), aflatoxin-B1 (1162658), 7,12-dimethylbenz(a)anthracene (57976), CD, CT and TS from 61% to 99%. The authors conclude that chlorophyllin is an antigenotoxic agent, effectively inhibiting the mutagenicity of the test mixtures.
Keywords
Mutagens; Airborne-particles; Coal-dust; Diesel-emissions; Tobacco-smoke; Alcoholic-beverages; Vitamins; Polycyclic-aromatic-hydrocarbons; Microbial-test-systems
CAS No.
50-32-8; 70-25-7; 56-49-5; 1162-65-8; 57-97-6
Publication Date
19940101
Document Type
Book or book chapter
Editors
Huang-M-T
Fiscal Year
1994
NTIS Accession No.
NTIS Price
ISBN No.
9780841227682
Issue of Publication
546
NIOSH Division
DRDS
Source Name
Food Phytochemicals for Cancer Prevention, I. Fruits and Vegetables
State
WV
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