Clastogenic and aneuploidogenic effects of cigarette smoke condensate, mitomycin C and vincristine sulfate.
Mutagenesis 1992 Nov; 7(6):457-460
Immunofluorescent staining of kinetochores (KC) in cytokinesis blocked Chinese-hamster-V79 cells fixed in-situ followed by chemical treatment was used to determine the clastogenic and aneuploidogenic effects of cigarette smoke condensate (CSC) as a test compound and mitomycin-C (50077) and vincristine-sulfate (2068782) as model compounds. The findings demonstrated that high concentrations of CSC are toxic to V79 cells. CSC was effective in the induction of micronucleus (MN) formation in this cell line. Data from immunofluorescent staining of MN indicated that CSC induces both KC+ and KC- micronucleated cells in a dose dependent fashion, suggesting that CSC contains both clastogenic and aneuploidogenic factors. The higher incidence of KC+ micronucleated cells compared to KC- micronucleated cells in the total micronucleated cells suggested a significant aneuploidy inducing effect of CSC. The authors note, however that since there are over 3,800 chemicals including at least 40 known carcinogens in tobacco smoke, it will not be easy to determine what chemical or chemical classes are causing clastogenic and aneuploidogenic activities.
NIOSH-Author; Tobacco-smoke; Clastogens; DNA-damage; Cytotoxic-effects; Cell-cultures; Mammalian-cells; Genotoxic-effects; Combustion-products; In-vitro-study