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Pharmacologic characterization of wool dust extract in isolated guinea pig trachea.

Authors
Schachter-EN; Zuskin-E; Buck-MG; Maayani-S; Marom-Z; Goswami-S; Rienzi-N
Source
Environ Res 1995 May; 69(2):90-95
NIOSHTIC No.
00232238
Abstract
The airway effects of wool dust were examined in guinea-pig tracheal rings and compared with previous results obtained for cotton bract extract (CBE). Wool dust was collected from carding machines at a mill in Croatia. Wool dust extract (WDE) was prepared by incubating wool with sterile distilled water in a weight to volume ratio of 1 gram wool dust to 10 milliliters sterile water for 24 hours. Tracheal tissue came from male albino-Hartley-guinea-pigs. Four segments each 4 to 6 millimeters wide, were cut from a single trachea and suspended between two L-shaped stainless steel hooks in an organ chamber. Dose response curves to WDE were measured in 30 guinea-pig tracheal rings obtained from different animals and expressed as a percentage of maximal carbachol contraction. WDE produced bronchoconstriction defined by a dose response curve similar to CBE. The maximal muscle tension for WDE was 57.9%, compared with 42.3% for CBE. Pyrilamine partially blocked the constricting effect of WDE at the highest WDE doses. Verapamil and trimethoxybenzoic-acid completely blocked the response to WDE. In contrast, CBE induced bronchoconstriction was significantly inhibited by a number of chemical agents, including atropine, indomethacin, and pyrilamine. Endotoxin was not present in the WDE. The authors conclude that WDE may have clinical effects related to non immunoglobulin-E mechanisms, which could have therapeutic implications for respiratory symptoms in wool textile workers.
Keywords
NIOSH-Publication; NIOSH-Grant; Pulmonary-system-disorders; Laboratory-animals; Textile-mills; Respiratory-hypersensitivity; In-vitro-study; Wools
Contact
Work Environment Univ of Lowell Research Fdn 450 Aiken St Lowell, MA 01854
CODEN
ENVRAL
Publication Date
19950501
Document Type
Journal Article
Funding Amount
209904.00
Funding Type
Grant
Fiscal Year
1995
NTIS Accession No.
NTIS Price
Identifying No.
Grant-Number-R01-OH-02953
Issue of Publication
2
ISSN
0013-9351
Priority Area
Pulmonary-system-disorders
Source Name
Environmental Research
State
MA
Performing Organization
University of Massachusetts Lowell, Lowell, Massachusetts
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