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Specific binding of (3H)methylscopolamine to glass gibre gilters.

Authors
Costa-LG
Source
J Pharm Pharmacol 1987 Mar; 39(3):239-240
NIOSHTIC No.
00232176
Abstract
The binding of tritium labeled methylscopolamine (MS) to glass fiber filters was investigated. The amount of binding was dependent on the buffer used for the incubation. When atropine was present, total binding was decreased 50 to 60%. Specific binding was noted in the presence of scopolamine, methylscopolamine, methylatropine, carbachol, oxotremorine, and acetylcholine. Total binding increased with increasing concentrations of MS. Specific binding to glass fiber filters was saturable. Presoaking the glass fiber filters in bovine serum albumin solution decreased total binding 45 to 60%, and specific binding 70 to 85%. Pretreatment of the filters with poly-L- lysine reduced total binding to glass fiber filters by more than 80% and virtually abolished specific binding. The author suggests that these findings indicate the presence on glass fiber filters of a binding site for MS. The author urges caution when MS is used as a muscarinic ligand and that measures to reduce filter binding or alternative separation procedures should be considered.
Keywords
NIOSH-Publication; NIOSH-Grant; Grants-other; Analytical-methods; Chemical-analysis; Analytical-chemistry; Filter-materials; Filtration;
Contact
Environmental Health University of Washington Dept of Environ Hlth, SC-34 Seattle, WA 98195
CODEN
JPPMAB
Publication Date
19870301
Document Type
Other
Funding Amount
97100
Funding Type
Grant
Fiscal Year
1987
NTIS Accession No.
NTIS Price
Identifying No.
Grant-Number-K01-OH-00054
Issue of Publication
3
ISSN
0022-3573
Priority Area
Other Occupational Concerns; Grants-other
Source Name
Journal of Pharmacy and Pharmacology
State
WA
Performing Organization
University of Washington, Seattle, Washington
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