Identification of a major human urinary metabolite of alachlor by LC- MS/MS.
Driskell-WJ; Hill-RH Jr.; Shealy-DB; Hull-RD; Hines-CJ
Bull Environ Contam Toxicol 1996 Jun; 56(6):853-859
Urinary metabolites of the herbicide alachlor (15972608) were identified in occupationally exposed subjects by liquid chromatography coupled to mass spectrometry. Urine samples were collected from four workers; daughter and parent scanning modes were then utilized to identify alachlor metabolites. On the basis of mass spectrometry and chromatographic behavior, the major metabolite was identified as alachlor-mercapturate. A method was then developed to measured this metabolite in urine. In the four urinary samples, alachlor-mercapturate levels ranged from 25 to 62% of the concentration of the alachlor hydrolysis product, 2,6-diethylaniline (579668) (DEA). No other alachlor metabolites were positively identified. The authors conclude that identification of alachlor- mercapturate allows for the development of novel methods to assess alachlor exposure and enable more effective biomonitoring; a method based on measurement of this metabolite should prove to be simpler, safer, and more specific than the previously used DEA method.
NIOSH-Author; Occupational-exposure; Herbicides; Biological-monitoring; Analytical-methods; Chromatographic-analysis; Urinalysis; Urine-chemistry; Mass-spectrometry
Bulletin of Environmental Contamination and Toxicology