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A biological monitoring method for o-toluidine and aniline in urine using high performance liquid chromatography with electrochemical detection.

Authors
Brown-KK; Teass-AW; Simon-S; Ward-EM
Source
Appl Occup Environ Hyg 1995 Jun; 10(6):557-565
NIOSHTIC No.
00227370
Abstract
A method for the determination of o-toluidine (95534) and aniline (62533) in the urine of workers occupationally exposed to amines was described. Urine samples were hydrolyzed by sodium-hydroxide, extracted with butyl-chloride and hydrochloric-acid, and the concentrations of the two amines in the aqueous phases were determined by high performance liquid chromatography and colorimetric electrochemical detection. The average recoveries of o- toluidine and aniline from field samples were 91% and 100%, the precision was 13% and 16% relative standard deviation, and the limits of detection were 0.6 and 1.4 micrograms/liter, respectively. Method performance was assessed by analyzing 171 specimens acquired during the field investigation. Analysis of urine specimens from chemical factory workers by this method demonstrated median preshift values for o-toluidine for exposed workers of 11 micrograms/liter and for nonexposed workers of 0.7 micrograms/liters. The median postshift value for exposed workers was 65 micrograms/liter, while that for nonexposed workers was 2.6 micrograms/liters. The median values seen for aniline were 11 micrograms/liter for preshift exposed, 2.0 micrograms/liter for preshift nonexposed, 23 micrograms/liter for postshift exposed, and 3.2 micrograms/liter for postshift nonexposed. The ruggedness of the method was tested by variations of eight procedural steps. The authors conclude that a significant uptake and accumulation of these amines occurred during and across workshifts based on the results obtained from the method.
Keywords
NIOSH-Author; Occupational-exposure; Biological-monitoring; Urinalysis; Analytical-methods; Laboratory-techniques; Chemical-analysis; Aromatic-hydrocarbons; Chemical-industry-workers
CODEN
AOEHE9
CAS No.
95-53-4; 62-53-3
Publication Date
19950601
Document Type
Journal Article
Fiscal Year
1995
NTIS Accession No.
NTIS Price
Issue of Publication
6
ISSN
1047-322X
Source Name
Applied Occupational and Environmental Hygiene
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