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erations of histone phosphorylation in rat spleen cells after treatment with the aromatic amine, 4,4'-methylene-bis(2-chloroaniline).

Authors
DeBord-DG; Cheever-KL; Booth-Jones-AD; Swearengin-TF; Savage-RE Jr.
Source
J Biochem Toxicol 1995 Feb; 10(1):19-23
NIOSHTIC No.
00226575
Abstract
A study was conducted on the effects of 4,4'-methylene-bis-(2- chloroaniline) (95534) (MOCA) on histone phosphorylation in rodent spleen cells. Spleen cells isolated from Sprague-Dawley-rats were incubated with up to 50 micromolar (microM) MOCA and the five major histone groups were evaluated using polyacrylamide gel electrophoresis. A significant increase in histone phosphorylation was seen following exposure to 10microM MOCA. MOCA incubation resulted in an initial transient decrease in histone phosphorylation followed by stimulation. The histone H2B group was least affected of all the histones to MOCA induced alterations. The degree of phosphorylation was unaffected by increasing MOCA concentrations with higher concentrations resulting in normal histone phosphorylation values for all histone groups with the exception of H3, which remained elevated following exposure to concentrations greater than 10microM.
Keywords
NIOSH-Author; Carcinogens; Anilines; Chlorine-compounds; Amines; Protein-biochemistry; Oxidative-phosphorylation; Arylamines
CODEN
JBTOEB
CAS No.
95-53-4
Publication Date
19950201
Document Type
Journal Article
Fiscal Year
1995
NTIS Accession No.
NTIS Price
Issue of Publication
1
ISSN
0887-2082
NIOSH Division
DBBS
Source Name
Journal of Biochemical Toxicology
State
OH
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