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Polycyclic aromatic hydrocarbon-DNA and protein adducts in coal tar treated patients and controls and their relationship to glutathione S-transferase genotype.

Authors
Santella-RM; Perera-FP; Young-TL; Zhang-J; Chiamprasert-S; Tang-D; Wang-LW; Beachman-A; Lin-H; DeLeo-VA
Source
Mutat Res 1995 Apr; 334(2):117-124
NIOSHTIC No.
00226199
Abstract
A panel of immunoassays designed to monitor exposure to polycyclic aromatic hydrocarbons (PAH) was evaluated by testing blood samples from coal tar treated patients. Blood samples obtained from coal tar treated psoriasis patients and untreated healthy referents were analyzed using a competitive enzyme linked immunosorbent assay (ELISA) for the detection of DNA and protein adducts with fluorescence endpoint detection, a competitive ELISA with color endpoint detection, and a noncompetitive ELISA for the detection of antibenzo(a)pyrene-diol-epoxide adducts. Elevated levels of PAH-DNA adducts were identified in coal tar treated patients, as determined by ELISA, compared with referents. No associations were seen between days of treatment prior to sample collection or smoking. No differences were seen in level of PAH-albumin adducts between patients and referents. In addition, no relationship could be demonstrated between the genotype for glutathione-S-transferase-M1 and DNA or protein adduct levels. Both patients and referents demonstrated similar titers of antiPAH-DNA adduct antibodies using the noncompetitive ELISA. The authors conclude that an ELISA measuring DNA adducts in white blood cells is the most sensitive method for detecting PAH exposure.
Keywords
NIOSH-Publication; NIOSH-Grant; Grants-other; Laboratory-techniques; Analytical-methods; DNA-adducts; Polycyclic-aromatic-hydrocarbons; Coal-tar; Pyrenes; Biological-monitoring; Immunological-tests; Blood-samples; Author Keywords: Coal tar; ELISA; DNA adducts; Protein adducts; Benzo[a]pyrene
Contact
Environmental Sciences Columbia University 650 West 168Th Street New York, NY 10032
CODEN
MUREAV
Publication Date
19950401
Document Type
Journal Article
Funding Amount
651727
Funding Type
Grant
Fiscal Year
1995
NTIS Accession No.
NTIS Price
Identifying No.
Grant-Number-R01-OH-02622
Issue of Publication
2
ISSN
0027-5107
Priority Area
Other Occupational Concerns
Source Name
Mutation Research
State
NY
Performing Organization
Columbia University New York, New York, New York
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