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Collagen mRNA content and distribution in the lungs of rats exposed to ozone.

Authors
Armstrong-LC; Watkins-K; Pinkerton-KE; Last-JA
Source
Am J Respir Cell Mol Biol 1994 Jul; 11(1):25-34
NIOSHTIC No.
00226161
Abstract
The effect of ozone (10028156) on collagen messenger RNA (mRNA) content and distribution was assessed in rat lung. Adult Sprague-Dawley-rats were exposed to either filtered air or 1.2 parts per million ozone for 7 days; rats were killed within 1 hour of the end of the exposure period for determination of mRNA content as well as collagen and total protein synthesis rates. The alpha1(I) procollagen mRNA concentration was significantly increased in the lungs of rats exposed to ozone in comparison to controls; the concentrations of the mRNAs for alpha1(III) procollagen and for beta-actin, however, were not significantly changed. The synthesis of type-I collagen in the lungs of rats exposed to ozone was 142% greater than in controls; synthesis of type-III collagen, in contrast, apparently increased by only 24%. Sites of increased expression of the alpha1(I) procollagen mRNA were localized via in-situ hybridization of paraffin embedded tissue sections; increased amounts of alpha1(I) procollagen mRNA were detected in the septal tips and peribronchiolar regions of ozone exposed animals. In addition, an apparent diffuse increase in alpha1(III) procollagen mRNA expression was observed. The authors conclude that exposure of rats to high concentrations of ozone causes an increase in the lung content of mRNA for type-I collagen.
Keywords
Laboratory-animals; Lung-cells; Genotoxic-effects; Toxic-gases; Cell-damage; Pulmonary-system; Protein-synthesis; Lung-fibrosis; Pollutants
CODEN
AJRBEL
CAS No.
10028156
Publication Date
19940701
Document Type
Journal Article
Funding Amount
1055222
Funding Type
Cooperative Agreement
Fiscal Year
1994
NTIS Accession No.
NTIS Price
Identifying No.
Cooperative-Agreement-Number-U07-CCU-906162
Issue of Publication
1
ISSN
1044-1549
Source Name
American Journal of Respiratory Cell and Molecular Biology
State
CA
Performing Organization
University of California - Davis
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