Skip directly to search Skip directly to A to Z list Skip directly to page options Skip directly to site content

NIOSHTIC-2 Publications Search

Search Results

Susceptibility to induction of chromosomal damage by metabolites of 1,3-butadiene and its relationship to 'spontaneous' sister chromatid exchange frequencies in human lymphocytes.

Authors
Wiencke-JK; Kelsey-KT
Source
Butadiene and Styrene: Assessment of Health Hazards. Sorsa M; Peltonen K; Vainio H; Hemminki K, eds., Lyon, France: International Agency for Research on Cancer, 1993 Jan; :265-273
Link
NIOSHTIC No.
00225623
Abstract
An in-vitro method for the examination of potential individual variations in the induction of chromosomal damage by diepoxybutane (298180) in cultured peripheral blood lymphocytes was developed. A group of 173 healthy workers from four work populations was studied over a 3 year period. Blood samples were obtained from the subjects. Demographic data, age, occupation, medical history, and prior/present exposure to agents which could affect the sister chromatid exchange (SCE) assay were assessed via questionnaire. Work history and exposure to possible genotoxicants at the workplace from industrial hygiene sampling results were obtained. Cell cultures from blood lymphocytes were established and treated with 6 micromolar diepoxybutane, monoepoxybutene (930223), or nitrogen- mustard. After 24 hours, the cultures were treated with bromodeoxyuridine, processed, and stained differentially for the analysis of SCEs and chromosomal aberrations. A bimodal distribution of SCEs was observed. Approximately 20% of the subjects were twice as sensitive to SCE induction as the remaining population. Lymphocytes from sensitive individuals had nearly four times more chromatid deletions, 6.2 times more isochromatid deletions, and 19 times more chromatid exchanges induced by diepoxybutane. Diepoxybutane sensitive individuals were also observed to have higher frequencies of uninduced SCEs. Lymphocytes sensitive to diepoxybutane induced SCEs were shown to have a normal sensitivity to nitrogen-mustard and a significantly increased sensitivity to monoepoxybutene. The authors suggest that the measurement of SCEs induced by diepoxybutane is a possible biomarker of sensitivity to butadiene (106990) genotoxicity and may be effective in occupational epidemiological studies.
Keywords
NIOSH-Publication; NIOSH-Grant; Cancer; Butadienes; Blood-cells; Chromosome-damage; Occupational-exposure; Epidemiology; DNA-damage; Genotoxic-effects
Contact
Environmental Health Harvard School of Public Hlth 665 Huntington Avenue Boston, MA 02115
CODEN
IARCCD
CAS No.
298-18-0; 930-22-3; 106-99-0
Publication Date
19930101
Document Type
Conference/Symposia Proceedings
Editors
Sorsa-M; Peltonen-K; Vainio-H; Hemminki-K
Funding Amount
162000
Funding Type
Grant
Fiscal Year
1993
NTIS Accession No.
NTIS Price
Identifying No.
Grant-Number-K01-OH-00110
Priority Area
Cancer
Source Name
Butadiene and Styrene: Assessment of Health Hazards
State
MA
Performing Organization
Harvard University, Boston, Massachusetts
TOP