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Urinary biomonitoring for alachlor exposure in commercial pesticide applicators by immunoassay.

Authors
Biagini-RE; Tolos-W; Sanderson-WT; Henningsen-GM; MacKenzie-B
Source
Bull Environ Contam Toxicol 1995 Feb; 54(2):245-250
NIOSHTIC No.
00224559
Abstract
A study was conducted to compare the results of an enzyme linked immunosorbent assay (ELISA) in the detection of the carcinogenic herbicide alachlor (15972608) in urine samples from alachlor exposed pesticide applicators against results obtained via the high performance liquid chromatography (HPLC) analyses of urine hydrolysate for diethylaniline (91667) (DEA). Three urine specimens (prework, postwork and morning after) were collected over a 24 hour period from each of 27 male pesticide applicators and mixers, then analyzed according to the appropriate procedure. The two analytical methods gave statistically significant different results when applied to the specimens that were above the analytical LOD for both methods. The mean result of samples tested by ELISA was 22.5+/-1.79 micromoles/liter alachlor or alachlor equivalents; HPLC mean result was 3.23+/-0.38 micromoles/liter DEA. The two methods showed a significant linear association when subjected to simple orthogonal regression analysis. The authors conclude that, despite unique sensitivity and potential cross reactivity, ELISA methods are a valid addition to instrument based analyses in screening for occupational alachlor exposure. ELISA methods have benefits in comparison to instrumental methods with regards to requiring smaller sample size, time of analysis, cost, sample throughput, and portability.
Keywords
NIOSH-Author; Agricultural-workers; Occupational-exposure; Herbicides; Urine-chemistry; Chromatographic-analysis; Analytical-methods; Biological-monitoring
CODEN
BECTA6
CAS No.
15972-60-8; 91-66-7
Publication Date
19950201
Document Type
Journal Article
Fiscal Year
1995
NTIS Accession No.
NTIS Price
Issue of Publication
2
ISSN
0007-4861
Source Name
Bulletin of Environmental Contamination and Toxicology
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