Relative release of interleukin-1beta and interleukin-1 receptor antagonist by alveolar macrophages. A study in asbestos-induced lung disease, sarcoidosis, and idiopathic pulmonary fibrosis.
Kline-JN; Schwartz-DA; Monick-MM; Floerchinger-CS; Hunninghake-GW
Chest 1993 Jul; 104(1):47-53
Release of interleukin-1beta (IL1b) and interleukin-1-receptor antagonist (IL1ra) by alveolar macrophages from patients with asbestos (1332214) induced lung diseases, sarcoidosis, and idiopathic pulmonary fibrosis (IPF) was examined. The study group consisted of ten healthy volunteers, 14 patients with asbestosis, 16 patients with asbestos related pleural disease (APD), 15 patients with sarcoidosis, and 15 patients with IPF. Bronchoalveolar lavage was performed. Lavagate cellularity was determined. The alveolar macrophages were recovered from the lavage fluid and assayed for spontaneous release of IL1ra and IL1b utilizing immunochemical methods. The data were compared with the smoking habits of the subjects. The sarcoidosis patients had significantly higher proportions of lymphocytes and lower proportions of macrophages than the healthy subjects. The IPF patients had significantly higher relative proportions of neutrophils and eosinophils and lower proportions of macrophages than the healthy subjects. Macrophages from the asbestosis, APD, sarcoidosis, and IPF patients released significantly more IL1b than the normal subjects. Release of IL1ra was significantly decreased in the APD, sarcoidosis, and IPF patients. Smoking was significantly associated with elevated lavagate cell counts and decreased release of IL1ra and IL1b. The IL1b/IL1ra concentration ratio (IL-1AI) was significantly associated with the observed increases in lavagate cellularity. The authors conclude that spontaneous release of IL1b and IL1ra from alveolar macrophages is altered by the presence of interstitial lung diseases such as asbestosis, sarcoidosis, and IPF. IL-1AI is a useful parameter for comparing cytokine activity in patients with interstitial lung disease.
NIOSH-Grant; Pulmonary-system-disorders; Asbestos-workers; Alveolar-cells; Immune-reaction; Clinical-techniques; Cigarette-smoking; Lung-fibrosis; Occupational-medicine
Internal Medicine University of Iowa Pulmonary Disease Division Iowa City, IA 52242
Pulmonary System Disorders
University of Iowa, Iowa City, Iowa