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Chromium Distribution and Toxicity in Mammalian Cells.

Authors
Uyeki-EM
Source
Department of Pharmacology/Toxicology/Therapeutics, Kansas University Medical Center, Kansas City, Kansas 1992 Feb:21 pages
Link
NIOSHTIC No.
00214054
Abstract
A final performance report on intracellular chromium (7440473) distribution and toxicity in mammalian cells was presented. Intracellular distribution and reactivity of radiolabeled chromium in Chinese-hamster cells was assessed by fractional centrifugation and a combination of anion exchange and ion pair high performance liquid chromatography. Greater than 75% of the radioactivity was localized in the cytosolic fraction. Glutathione, several nucleotides, and an unidentified metalloprotein or peptide were found to interact with chromate or its reduced derivative. In cultured mouse cells, 10 micromolar chromium(VI) irreversibly inhibited DNA synthesis to 50% of control values. Chromosomal, cytochemical, and biochemical analyses revealed that chromium(VI) was more cytotoxic than chromium(III). A comprehensive computer aided imaging system developed to study the in-vivo toxic effects of chromium showed that chromium caused regional toxicity in the liver of mice which primarily affected cells in the periportal region. The author concludes that chromate's toxicity appears to result from its ability to oxidize substances such as glutathione within the cell, thereby producing ions which have the ability to coordinate with biomolecules.
Keywords
NIOSH-Grant; Grants-other; Mammalian-cells; Cell-cultures; Cell-damage; Chromium-compounds; Toxic-effects; Cytotoxic-effects; Laboratory-animals; Biochemical-tests;
Contact
Pharmacology University of Kansas 39Th St at Rainbow Blvd Kansas City, Kans 66103
CAS No.
7440-47-3;
Publication Date
19920201
Document Type
Final Grant Report;
Funding Amount
713490.00
Funding Type
Grant;
Fiscal Year
1992
NTIS Accession No.
NTIS Price
Identifying No.
Grant-Number-R01-OH-01630
NIOSH Division
OEP
Priority Area
Other Occupational Concerns; Grants-other;
Source Name
Department of Pharmacology/Toxicology/Therapeutics, Kansas University Medical Center, Kansas City, Kansas
State
KS;
Performing Organization
University of Kansas Col Hlth Sci & Hosp, Kansas City, Kansas
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