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Computer-based image analysis of cartilage differentiation in embryonic limb bud micromass cultures.

Authors
Garrison-JC; Peterson-P; Uyeki-EM
Source
J Microsc 1989 Dec; 156(Pt 3):353-361
NIOSHTIC No.
00214033
Abstract
An analysis was made of the effects of benzamide (55210) (BAM), sodium-butyrate (156547) and dimethyl-sulfoxide (67685) (DMSO) on chondrogenesis in embryonic chick limb bud cells in culture using a computer imaging system. A micromass culture technique was used to prepare the cultures from stage 23/24 White-Leghorn-chicken embryos. There was a direct relationship between the degree of chondrogenesis and the amount of Alcian-blue staining. Low magnification images of individual micromass colonies were converted into derived images based on their percentage transmittance relative to the background. The derived images were analyzed for relative degree of chondrogenesis using as descriptors the area and integrated optical density (IOD). IOD was the preferred descriptor due to its accuracy over a wide range of threshold values. BAM markedly enhanced chondrogenesis in culture. Sodium-butyrate markedly inhibited chondrogenesis and DMSO produced mixed results. The authors suggest that the same descriptors may be used in quantifying data from various objects which can be stained in a selected and quantitative fashion.
Keywords
NIOSH-Publication; NIOSH-Grant; Grants-other; Amides; Cell-cultures; Cytotoxic-effects; Developmental-disorders; Skeletal-system-disorders; In-vitro-studies; Author Keywords: Cartilage; computer; chick; image analysis; embryo; micromass
Contact
Pharmacology University of Kansas 39Th St at Rainbow Blvd Kansas City, Kans 66103
CODEN
JMICAR
CAS No.
55-21-0; 156-54-7; 67-68-5
Publication Date
19891201
Document Type
Journal Article
Funding Amount
713490
Funding Type
Grant
Fiscal Year
1990
NTIS Accession No.
NTIS Price
Identifying No.
Grant-Number-R01-OH-01630
ISSN
0022-2720
Priority Area
Other Occupational Concerns; Grants-other
Source Name
Journal of Microscopy
State
KS
Performing Organization
University of Kansas Col Hlth Sci & Hosp, Kansas City, Kansas
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