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Purification and characterization of cytochrome P450 isozymes from /beta-naphthoflavone-induced adult hen liver.

Authors
Gupta-RP; Lapadula-DM; Abou-Donia-MB
Source
Arch Biochem Biophys 1990 Jan; 282(1):170-182
NIOSHTIC No.
00214021
Abstract
Cytochrome-P450 isozymes induced in hens by a classic polycyclic aromatic hydrocarbon beta-naphthoflavone (6051872) were isolated and characterized. Adult leghorn-hens were injected intraperitoneally with 2% beta-naphthoflavone at 80mg/kg daily for 4 days; 24 hours after the last dose, hens were killed, and the liver microsomes were isolated. The isolation procedure was a modification of the method used for purifying cytochrome-P450 isozymes from phenobarbital (PB) induced hen livers. Cytochromes-P450 beta-NF-A1/beta-NF-A2 were identical or very similar to PB induced PB-A, as all of them exhibited almost the same amino terminal sequence, mobilities during one and two dimensional electrophoresis, and other properties. The other two immunochemically related cytochrome-P450s, beta-NF-B and beta-NF-C, were distinct isozymes with different N-terminal amino acid sequences, and several biophysical and biochemical properties. The amino terminal sequences of cytochrome-P450 beta-NF-B and beta- NF-C were also different from other cytochrome-P450s purified from mammalian or nonmammalian species. The authors conclude that purification of these P450s and availability of antiserums will be useful in examining their role in the metabolism of various environmental neurotoxic compounds.
Keywords
NIOSH-Publication; NIOSH-Grant; Neurotoxic-effects; Enzyme-activity; Nervous-system-disorders; Laboratory-animals; Liver-microsomal-enzymes; Chemical-structure
Contact
Pharmacology Duke University Department of Pharmacology Durham, N C 27710
CODEN
ABBIA4
CAS No.
6051-87-2;
Publication Date
19900101
Document Type
Journal Article
Funding Amount
1567389
Funding Type
Grant
Fiscal Year
1990
NTIS Accession No.
NTIS Price
Identifying No.
Grant-Number-R01-OH-00823
Issue of Publication
1
ISSN
0003-9861
Priority Area
Neurotoxic Disorders; Neurotoxic-effects
Source Name
Archives of Biochemistry and Biophysics
State
NC
Performing Organization
Duke University, Durham, North Carolina
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