Application of Raney nickel to measure adducts of styrene oxide with hemoglobin and albumin.
Rappaport-SM; Ting-D; Jin-Z; Yeowell-O'Connell-K; Waidyanatha-S; McDonald-T
Chem Res Toxicol 1993 Mar; 6(2):238-244
The measurement of adducts of styrene-7,8-oxide (96093) (SO) with hemoglobin and albumin proteins using Raney-nickel (Ra-Ni) was described. Hemoglobin and albumin were isolated from human or rat whole blood with or without prior incubation with SO, or from Sprague-Dawley-rats treated 24 hours earlier with styrene (100425) or SO. Analysis of hemoglobin and albumin proteins from blood samples incubated with SO using the Ra-Ni procedure demonstrated that 6.0% of the binding to hemoglobin and 76.4% of the binding to albumin were accounted for. Pseudo first order behavior was observed in the evaluation of the elimination of SO from rat and human blood. The efficiency of cleavage using Ra-Ni was evaluated in samples containing the model compounds 4-(methylthio)benzyl-alcohol and benzylmethyl-sulfide in hemoglobin digests. Second order rate constants calculated for the reactivity of SO with the blood proteins indicated that SO was most reactive with rat hemoglobin and rat albumin followed by human albumin, and finally, human hemoglobin. Treatment with SO resulted in a greater production of protein adducts than did treatment with styrene.
NIOSH-Publication; NIOSH-Grant; Grants-other; Metabolic-study; Organic-solvents; Analytical-chemistry; Hemoproteins; Laboratory-animals; Blood-analysis
Biomedical & Environ Hlth Scis University of California School of Public Health Berkeley, CA 94720
Other Occupational Concerns; Grants-other
Chemical Research in Toxicology
University of California Berkeley, Berkeley, California