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Effect of phorbol and bryostatin I on chondrogenic expression of chick limb bud, in vitro.

Authors
Garrison-JC; Pettit-GR; Uyeki-EM
Source
Life Sci 1987 Oct; 41(17):2055-2061
NIOSHTIC No.
00213644
Abstract
The effect of phorbol-12-myristate-13-acetate (16561298) (PMA) on chondrogenesis was studied in chick limb bud cells. The effect of bryostatin-I (83314016) was examined for comparison purposes. Limb bud cells harvested from stage 23 or 24 White-Leghorn-chick embryos were incubated with up to 10(-6) molar (M) PMA or 10(-7)M bryostatin- I alone or in combination for up to 96 hours. The effects on cell proliferation were determined using a Coulter channelyzer. The effects on chondrogenesis were assessed by determining the extent of cartilage nodule formation and Alcian-blue dye staining for chondroitin-sulfate. Four days' exposure to 10(-7)M or higher PMA caused a significant decrease in cartilage nodule formation and the amount of Alcian-blue staining. No effects on cell proliferation were observed. When examined according to incubation time, the greatest inhibition of chondrogenesis occurred after 49 to 96 hours of PMA treatment. Bryostatin-I had a slight stimulatory effect on cartilage nodule dye formation and Alcian-blue staining. Coincubation of 10(-7)M bryostatin-I with 10(-7)M PMA abolished the inhibitory effect of PMA on chondrogenesis. The authors conclude that the inhibitory effect of PMA on chondrogenesis is due to a suppression of chondrocyte phenotypic expression rather than cell commitment to chondrocytic expression. Since both PMA and bryostatin-I are protein-C-kinase activators, the mechanism of PMA induced chondrogenesis inhibition must involve processes other than protein-C-kinase activation.
Keywords
NIOSH-Publication; NIOSH-Grant; Grants-other; Biokinetics; Histochemical-analysis; Dose-response; Enzyme-activity; In-vitro-studies; Laboratory-animals; Growth-inhibition
Contact
Pharmacology University of Kansas 39Th St at Rainbow Blvd Kansas City, Kans 66103
CODEN
LIFSAK
CAS No.
16561-29-8; 83314-01-6
Publication Date
19871001
Document Type
Journal Article
Funding Amount
713490.00
Funding Type
Grant
Fiscal Year
1988
NTIS Accession No.
NTIS Price
Identifying No.
Grant-Number-R01-OH-01630
Issue of Publication
17
ISSN
0024-3205
Priority Area
Grants-other
Source Name
Life Sciences
State
KS
Performing Organization
University of Kansas Col Hlth Sci & Hosp, Kansas City, Kansas
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