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High-pressure liquid chromatography of neurotoxic phenylphosphonothioate esters and related compounds.

Authors
Lasker-JM; Sivarajah-K; Eling-TE; Abou-Donia-MB
Source
Anal Biochem 1980 Dec; 109(2):369-375
NIOSHTIC No.
00213641
Abstract
A chromatographic method for determining neurotoxic phenylphosphonothioate esters was developed. Five to 25 microliter samples containing leptophos (21609905), O-2,5-dichlorophenyl-O- methyl-phenylphosphonothioate (53490781) (desbromoleptophos), O- ethyl-O-4-nitrophenylphenylphosphonothioate (2104645) (EPN), cyanofenphos (13067931), and O-ethyl-O-2,4-dichlorophenyl- phenylphosphonothioate (3792594) (EPBP) were injected into a reversed phase high pressure liquid chromatography system containing a microparticulate RP-8 column and an ultraviolet detector operating at 280 nanometers. The mobile phase was varied from 1% methanol, 5% glacial-acetic-acid and 95% water to 95% methanol and 5% water over 30 minutes using a gradient elution technique following a 10 minute isocratic delay. Leptophos, desbromoleptophos, EPN, cyanofenphos, and EPBP were well separated from each other and from their degradation products, which included oxygen analogues and phenols. The detection limit for EPN was 0.01 microgram (microg). The detection limit for leptophos, cyanofenphos, desbromoleptophos, and EPBP was 0.1microg. The relationship between the detector response (peak area) and amounts injected was linear over at least a 100 fold range. The technique was applied to determining the metabolites formed when EPN was incubated with rat liver microsomes in the presence of NADPH. The authors conclude that the technique can rapidly determine leptophos, desbromoleptophos, EPN, cyanofenphos, and EPBP. Only a single chromatographic step is required to perform the analysis.
Keywords
NIOSH-Publication; NIOSH-Grant; Neurotoxic-effects; Analytical-methods; Organo-phosphorus-pesticides; Chromatographic-analysis; Biotransformation; Liver-microsomes
Contact
Pharmacology Duke University Department of Pharmacology Durham, N C 27710
CODEN
ANBCA2
CAS No.
21609-90-5; 53490-78-1; 2104-64-5; 13067-93-1; 3792-59-4
Publication Date
19801201
Document Type
Journal Article
Funding Amount
1567389
Funding Type
Grant
Fiscal Year
1981
NTIS Accession No.
NTIS Price
Identifying No.
Grant-Number-R01-OH-00823
Issue of Publication
2
ISSN
0003-2697
Priority Area
Neurotoxic Disorders; Neurotoxic-effects
Source Name
Analytical Biochemistry
State
NC
Performing Organization
Duke University, Durham, North Carolina
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