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Analysis of environmental histamine from agricultural dust.

Authors
Siegel-PD; Shahan-TA; Sorenson-WG
Source
Scand J Work, Environ & Health 1992 Jun; 18(Suppl 2):60-62
NIOSHTIC No.
00209515
Abstract
A method was developed for the quantitation of histamine (51456) levels from environmental air and bulk samples in the laboratory. The assay was applicable to monitoring of histamine exposure in the workplace. Representative samples of bulk materials to which the agricultural workers can be exposed were collected. Two extraction media, distilled water and perchloric-acid, were evaluated for the extraction of histamine from bulk and filter samples. The most reliable method developed involved perchloric-acid extraction followed by the butanol-acid clean up technique. This method cleaned the extract, removing possible interferences and concentrated the samples. Extracts were alkalinized with sodium- hydroxide and extracted into butanol. After a second extraction the total butanol extract was added to heptane and back extracted into hydrochloric-acid. An aliquot of the hydrochloric-acid was recovered and stored at minus 70 degrees-C until assayed. Hay, chicken coop bedding, rafter dust, wood mulch, silage, and cotton dust demonstrated measurable levels of histamine. Cultures of bacteria and fungi were tested for histamine production. Gram negative bacteria, gram positive bacteria, and fungi were found to produce histamine.
Keywords
NIOSH-Author; Agricultural-industry; Agricultural-workers; Bacterial-dusts; Airborne-dusts; Respiratory-system-disorders; Plant-dusts; Microorganisms
CODEN
SWEHDO
CAS No.
51-45-6
Publication Date
19920601
Document Type
Journal Article
Fiscal Year
1992
NTIS Accession No.
NTIS Price
ISSN
0355-3140
Priority Area
Respiratory-system-disorders
Source Name
Scandinavian Journal of Work, Environment and Health
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