Skip directly to search Skip directly to A to Z list Skip directly to page options Skip directly to site content

NIOSHTIC-2 Publications Search

Search Results

Inhibition of intercellular communication in human keratinocytes by fractionated asphalt fume condensates.

Authors
Wey-HE; Breitenstein-MJ; Toraason-MA
Source
Carcinogenesis 1992 Jun; 13(6):1047-1050
NIOSHTIC No.
00208497
Abstract
The effect of asphalt fume condensate fractions on gap junctional intercellular communication in a human epidermal keratinocyte cell line was investigated. Cultures of epidermal keratinocytes were exposed to medium containing one of five asphalt fume condensate fractions. Gap junction intercellular communication was assessed by the dye coupling method using fluorescent dye microinjection. The total number of dye positive adjacent cells were used as an indication of gap junction intercellular communication. Asphalt fume condensate fractions induced toxic effects that were dependent on the total mass of the added fraction. All the fractions demonstrated a concentration dependent inhibition of dye coupling, indicating an inhibition in gap junction intercellular communication. Cells exposed to some fractions of asphalt fume condensates exhibited significantly reduced DNA content following overnight exposure, but there were no indications of membrane damage or cell killing. The authors conclude that fractionated asphalt fume condensates may have tumor promoting activity by inducing an inhibition of gap junction intercellular communication.
Keywords
NIOSH-Author; Asphalt-fumes; Carcinogenesis; Carcinogens; Cell-damage; Cell-function; Cytotoxicity; In-vitro-study; Skin-tumors; Toxic-effects; Toxic-vapors
CODEN
CRNGDP
Publication Date
19920601
Document Type
Journal Article
Fiscal Year
1992
NTIS Accession No.
NTIS Price
Issue of Publication
6
ISSN
0143-3334
NIOSH Division
DBBS
Source Name
Carcinogenesis
State
OH
TOP