Skip directly to search Skip directly to A to Z list Skip directly to page options Skip directly to site content

NIOSHTIC-2 Publications Search

Search Results

Ortho-toluidine blood protein adducts: HPLC analysis with fluorescence detection after a single dose in the adult male rat.

Authors
Cheever-KL; DeBord-DG; Swearengin-TF; Booth-Jones-AD
Source
Fundam Appl Toxicol 1992 May; 18(4):522-531
NIOSHTIC No.
00207130
Abstract
High performance liquid chromatography (HPLC) and fluorescence detection were used to quantify ortho-toluidine (95534) (OT) blood protein adducts after single dose administration to adult male Sprague-Dawley-rats. The animals received labeled OT concentrations of 10, 20, 40, 50, and 100mg/ml corn-oil by gavage or intraperitoneal injection. Blood collected from treated animals was processed for isolation of hemoglobin and albumin. Mild alkaline hydrolysis of the labeled OT adducted proteins followed by extraction with ethyl-acetate yielded 63% of bound radioactivity. A single radiolabeled peak obtained on HPLC was identified as OT by gas chromatography/mass spectrometry. Fluorescence detection was used in subsequent experiments. After administration of a single 50mg/kg dose of OT, the adduct accumulated more in association with Hb than with albumin. A linear relationship was evident between dose and 4 hour adduct formation. The highest adduct levels were detected in animals dosed at 100mg/kg body weight. Serial termination of rats over 28 days revealed that the adduct half life is 11 days. The authors conclude that the quantification of OT-Hb adducts may provide a valuable index of cumulative long term exposure to this carcinogen.
Keywords
NIOSH-Author; Biokinetics; Carcinogens; Chemical-kinetics; Laboratory-animals; Metabolism; Organic-compounds; Toxicology; Chromatographic-analysis
CODEN
FAATDF
CAS No.
95-53-4
Publication Date
19920501
Document Type
Journal Article
Fiscal Year
1992
NTIS Accession No.
NTIS Price
Issue of Publication
4
ISSN
0272-0590
NIOSH Division
DBBS
Source Name
Fundamental and Applied Toxicology
State
OH
TOP