Skip directly to search Skip directly to A to Z list Skip directly to page options Skip directly to site content

NIOSHTIC-2 Publications Search

Search Results

Aerosolized neutral endopeptidase reverses ozone-induced airway hyperreactivity to substance P.

Authors
Murlas-CG; Lang-Z; Williams-GJ; Chodimella-V
Source
J Appl Physiol 1992 Mar; 72(3):1133-1141
NIOSHTIC No.
00206429
Abstract
Hartley-guinea-pigs were exposed to ozone (10028156) for 2 hours, and the effects on airway neutral endopeptidase (NEP) and reactivity to substance-P were studied. Intact, unanesthetized, spontaneously breathing male guinea-pigs were exposed to 3.0 parts per million ozone or to room air. Ozone exposure resulted in a substantial increase in airway reactivity to substance-P. Treatment with phosphoramidon caused a substantial increase in the substance-P reactivity of air exposed guinea-pigs. The values of NEP activities of tracheal homogenates from ozone exposed animals ranged from 16.2 to 20 nanomoles/minute milligram/protein and 22.7 to 27.5 in air exposed animals. In all animals tested, active NEP reduced substance-P reactivity after ozone exposure to preozone levels. Aerosolized phosphoramidon treatment following NEP reversed this finding. No significant influence on substance-P reactivity post ozone was demonstrated by heat inactivated NEP inhalation. A considerable decrease in substance-P reactivity produced by active NEP inhalation post ozone was noted. The authors suggest that an airway disorder associated with the loss of NEP activity can be reversed by inhaling an aerosol containing that enzyme.
Keywords
NIOSH-Publication; NIOSH-Grant; Pulmonary-system-disorders; Drug-therapy; Enzyme-activity; Lung-irritants; Laboratory-animals; Inhalation-studies; Airway-resistance; Bronchial-asthma; Respiratory-system-disorders
Contact
Rush-Presbyterian St. Luke Medical Center, Department of Medicine (Pulmonary), 1653 W. Congress Parkway, Chicago, IL 60612
CODEN
JAPHEV
CAS No.
10028-15-6
Publication Date
19920301
Document Type
Journal Article
Funding Amount
127961
Funding Type
Grant
Fiscal Year
1992
NTIS Accession No.
NTIS Price
Identifying No.
Grant-Number-K01-OH-00060
Issue of Publication
3
ISSN
8750-7587
Priority Area
Pulmonary-system-disorders
Source Name
Journal of Applied Physiology
State
IL
Performing Organization
Rush-Presbyterian St. Luke Medical Center, Department of Medicine (Pulmonary), Chicago, IL 60612
TOP